M52 - STELLARIS 8 CryoCLEM with Falcon FLIM

System description

The Leica STELLARIS 8 FALCON is an upright point laser scanning confocal microscope featuring Fluorescence Lifetime Imaging (FLIM) with FALCON technology. The system is equipped with White Light Laser (WWL), which provides flexible excitation across the wide spectrum. M52 has five sensitive HyD detectors and a XY-motorized stage with a Z-Galvo insert. The incubator enclosing the whole microscope allows both temperature and CO2 control. Upon conversion, the system can be configured for Cryo-CLEM experiments.

Widefield illumination

LED for brightfield

Lasers for confocal imaging

Diode 405 nm

OPSL 488 nm

OBIS 561 nm

Diode 638 nm

White Light Laser (WLL) 440 - 790 nm

Filters

2x HyDS detectors 

2x HyDX detectors (optimized for FLIM)

1x HyDR detector (optimized for FLIM)

T-PMT (transmitted light) detector

Objectives

10X/0.40 DRY HC PL APO CS2

20X/0.75 DRY HC PL APO CS2

25X/0.95 WATER HC FLUOTAR L VISIR

40X/1.25 GLYCEROL HC PL APO CS2

63X/1.40 OIL HC PL APO CS2

 

50X/0.90 CLEM HCX PL APO

Additional devices

Leica K5 camera

Cryo-CLEM equipment