M48 – LSM800 / Vertical
The M48 system (vertical microscope) is an inverted point laser scanning confocal microscope (LSM800) equipped with high-sensitive GaAsP (Gallium Arsenide, like LSM780 and 880) detectors and a transmitted light detector. It is a complete spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. This system can be used to acquire 2 different fluorophores simultaneously. A variable secondary dichroic (VSD) allows the precise splitting of the emitted wavelengths into 2 channels.
This system has been custom converted into a vertical configuration that facilitates imaging of plant seedlings in close to natural growth conditions (geotropism).
Implemented root tracking software allows following of the root-tip growth over time.
The system is running under ZEN Blue.
Colibri LED: 385nm, 475nm, 555nm, 630nm
LED Transmitted Light Illumination
Laser for Confocal Imaging
Laser Diode 405 5mW
Laser Diode 488 10mW
Laser Diode 561 10mW
Laser Diode 639 5mW
CH1 GaAsP: SP470, SP545, SP620 - detection range 380-650nm
CH2 GaAsP: LBF640, LP575, LP665, SP620 - detection range 490-750nm
ESSID (transmitted light)
VSD range - 420-630nm in 1nm steps
2,5x/0.085 EC Plan-Neofluar
5x/0.16 EC Plan-Neofluar
10x/0.3 EC Plan-Neofluar
20x/0.8 PlanApochromat DIC
40x/0.6 LD Plan-Neofluar
63x/0.95 EC Plan-Neofluar
Axiocam 705 mono
XY Scanning Stage