M30 - Pannoramic FLASH 250 III

System Description

The M30 system is an automated, extremely fast (2x faster than M28 in bright field), widefield microscope equipped with transmitted and fluorescent light source. It can be used to scan up to 250 slides in both transmitted and fluorescence. This system comes with darkfield preview mode which is used to recognize samples to be scanned in the fluorescence as well as in the transmitted light mode. The advantages of this system are the constant illumination and white balance as well as the elimination of the operator induced error. The system can acquire Z-stacks and deliver maximum intensity projection (MIP) important for 3D structures. 

As a result, you get images of the whole sample (and not only of the "preferentially" selected area) that can be subjected to automated image analysis. The system can run on its own, no attendance needed. 
Slides for the automatic scanner have to be prepared in a specific way. Please ask the BioOptics staff for directions or refer to fileadmin/additional_pages/core_facilities/biooptics/Protocols_Publication_etc/Mirax_slide_preparation.pdf
Protocols. This system is exclusively operated by the BioOptics facility staff. It helps to automate your experiments and fits into the chain of - automated staining - imaging - image analysis.

 

Find more information here.

Illumination

Lumencor Spectra 6 – Solid state light engine
WhiteLight LED for transmitted light

Filter

Multiband set
Ex 365/18nm   Em 397/18nm

Ex 485/26nm   Em 521/27nm

Ex 559/32nm   Em 607/40nm

Ex 649/19nm   Em 700/57nm


Singleband set
Ex 480/40nm   Em 535/50nm 

Ex 560/55nm   Em 645/75nm  

Ex 620/60nm   Em 700/75nm

Objectives

20/0.8 plan-apochromat

40/0.95 plan-apochromat

 

 

 

Camera

Adimec Quartz Q12A180 – transmitted light – pixel size on the image 0,242797µm (20x objective) and 0,1214µm (40x objective)

PCO.edge – fluorescence – pixel size on the image – 0,3250µm (only 20x objective)

 

 

 

Reservation

please contact BioOptics staff