Peptide synthesis

Peptide synthesis, purification of antibodies and other proteins

These services are provided by Mathias Madalinski and Zsuzsanna Muhari-Portik, located in the VBC 6 ground floor, room E 24, tel 3829.

Peptide synthesis

Standard peptide synthesis:

  • Up to 50aa length (strongly depending on sequence)
  • Usually, HPLC purified
  • Yield 10-50mg



  • Synthesis in 96well format (up to 96 peptides synthesized in parallel)
  • Up to 25aa length
  • Usually, not HPLC purified (only if absolutely required a small subset can be purified)
  • Crude purity approx. 70%  (depending on sequence and length)
  • Yield 100µg to 2mg


SPOT synthesis:

  • Up to 600 individual peptides can be synthesized on one Cellulose membrane (two membranes in parallel possible)
  • Approx. 20nmol per spot
  • synthesis works best for peptides with a length between 6aa and 15aa (longer peptides are possible, but purity will drop)
  • purity approx. 80%

 More detailed information can be found here


Synthesis of peptides carrying modifications or amino acids with heavy isotopes:

Modifications which are routinely available in our facility:

  • N-term acetylated
  • C-term amidated
  • Lys(me)
  • Lys(me2)
  • Lys(me3)
  • Lys(ac)
  • Ser(PO4)
  • Thr(PO4)
  • Tyr(PO4)
  • Tyr(SO3)
  • N-term Biotin (with aminohexanoic acid or 2xbAla spacer recommend)
  • N-term 5,(6) Carboxyfluorescein (isomer mix) (with aminohexanoic acid or 2xbAla spacer recommended)

Modifications that can be synthesized with components provided by the scientist:

  • Arg(Me)
  • Arg(Me2-Sym)
  • Arg(Me2-Asym)
  • N-term 5 or 6 Carboxyfluorescein (monoisomer) (with aminohexanoic acid or 2xbAla spacer recommed)
  • N-Term TAMRA (with aminohexanoic acid or 2xbAla spacer recommened)
  • C-term Biotin (with PEG5- or Ethyl Spacer)
  • C-term Carboxyfluorescein (monoisomer or isomer mix)
  • C-term TAMRA (with PEG5- or Ethyl Spacer)
  • Amino acids labeled with heavy isotopes (15N, 13C, 2H)


Other modifications/fluorophores might be available on request.


Purification of Antibodies:

  • affinity purification of polyclonal anti-peptide-antibodies using corresponding peptide columns
  • purification of polyclonal and monoclonal antibodies via protein A or protein G columns


Small scale RP-HPLC purifications (up to 1mg):

  • Oligos
  • fluorophore labeled molecules (Oligos, CoA, peptides…)

other purifications might be possible on request


TAQ purification (in cooperation with MBS) 

Other services

KLH conjugation of peptides for immunization of animals

Freeze drier