Peptide synthesis

These services are provided by Mathias Madalinski and Zsuzsanna Muhari-Portik, located in the VBC 6 ground floor, room E 24, tel 3829.

Standard peptide synthesis:

• Up to 50aa length (strongly depending on sequence)
• Usually, HPLC purified
• Yield 10-50mg

Tipsynthesis:

• Synthesis in 96well format (up to 96 peptides synthesized in parallel)
• Up to 25aa length
• Usually, not HPLC purified (only if absolutely required a small subset can be purified)
• Crude purity approx. 70%  (depending on sequence and length)
• Yield 100µg to 2mg

SPOT synthesis:

• Up to 600 individual peptides can be synthesized on one Cellulose membrane (two membranes in parallel possible)
• Approx. 20nmol per spot
• synthesis works best for peptides with a length between 6aa and 15aa (longer peptides are possible, but purity will drop)
• purity approx. 80%

 More detailed information can be found here

Synthesis of peptides carrying modifications or amino acids with heavy isotopes:

Modifications which are routinely available in our facility:

• N-term acetylated
• C-term amidated
• Lys(me)
• Lys(me2)
• Lys(me3)
• Lys(ac)
• Ser(PO4)
• Thr(PO4)
• Tyr(PO4)
• Tyr(SO3)
• N-term Biotin (with aminohexanoic acid or 2xbAla spacer recommend)
• N-term 5,(6) Carboxyfluorescein (isomer mix) (with aminohexanoic acid or 2xbAla spacer recommended)
  
  

Modifications that can be synthesized with components provided by the scientist:

• Arg(Me)
• Arg(Me2-Sym)
• Arg(Me2-Asym)
• N-term 5 or 6 Carboxyfluorescein (monoisomer) (with aminohexanoic acid or 2xbAla spacer recommed)
• N-Term TAMRA (with aminohexanoic acid or 2xbAla spacer recommened)
• C-term Biotin (with PEG5- or Ethyl Spacer)
• C-term Carboxyfluorescein (monoisomer or isomer mix)
• C-term TAMRA (with PEG5- or Ethyl Spacer)
• Amino acids labeled with heavy isotopes (15N, 13C, 2H)

Other modifications/fluorophores might be available on request.

Purification of Antibodies:

• affinity purification of polyclonal anti-peptide-antibodies using corresponding peptide columns
• purification of polyclonal and monoclonal antibodies via protein A or protein G columns

Small scale RP-HPLC purifications (up to 1mg):

• Oligos
• fluorophore labeled molecules (Oligos, CoA, peptides…)

other purifications might be possible on request

TAQ purification (in cooperation with MBS) 

KLH conjugation of peptides for immunization of animals

Freeze drier

MALDI