Sanger Sequencing

Applied Biosystems DNA Analyzer

The Sanger Sequencing Facility has 2 DNA sequencers, the ABI 3730xl (runs 96 samples simultaneously) and the ABI 3730 DNA Analyzer (runs 48 samples simultaneously). These instruments are capillary based, use Bid Dye chemistry and run on Data Collection software. Both are the Gold Standard in medium-to-high throughput genetic analysis. 


Main advantages to using the 3730 and 3730xl:

  • Short runtime: sequencing takes 2 hours/run and fragment analysis around 45 minutes/run
  • Fast run times for large number of samples
  • Great read lengths and quality scores
  • Little instrument maintenance is needed