F18 - Miltenyi Tyto Lux
System Description
The Tyto Lux is a benchtop, cartridge-based cell sorting system from Miltenyi Biotec that combines microfluidic, closed-system sorting with an emphasis on cell viability and sterility. The system is equipped with 4 lasers to perform label-based high-purity sorting without the high-pressure droplet formation used in traditional flow cytometers, therefore reducing shear stress and decompression.
Laser
405nm Violet Laser, 100mW
488nm Blue Laser, 100mW
552nm Green Laser, 100mW
638nm Red Laser, 100mW
Detection
V1: 448/20 nm - BFP, BV421, DAPI, Hoechst33342
V2: 520/36 nm - Alexa Fluor 430, BV510
V3: 579/34 nm - BV570
V4: 641/75 nm - BV650
B1: 520/36 nm - FITC, GFP, Alexa Fluor 488
B/Y2: 579/34 nm - PE, tdtomato
B/Y3: 615/20 nm - PE-Texas Red, mCherry
B/Y4: 679/75 nm - PE-Texas Red, mCherry, PE-Cy5
B/Y5: 785/62 nm - PE-Cy7
R1: 667/30 nm - APC, Alexa Fluor 647, iRFP670
R2: 725/40 nm - Alexa Fluor 700, iRFP720, DRAQ7
R3: 785/62 nm - APC-Cy7
Backscatter and side scatter for detection of size & granularity of cells