OMX - Structured Illumination Superresolution Microscope

System Description

DeltaVision OMX is based on the structured illumination method (SIM) that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit, delivering three-dimensional (XYZ) resolution doubling. This advanced microscope can also be used for super fast wide-field fluorescence acquisition.(Gustafsson et al. 2008) As a wide-field fluorescence microscope it is especially suited for flat samples with thickness up to ca. 20µm.

This microscope, although officially belonging to CSF, can be used by IMP-IMBA-GMI users as any other system of the BioOptics facility, without any extra costs.

Widefield Illumination

6 color InsightSSI™ illuminator with 405, 445, 488, 514, 568, 642 nm excitation wavelengths

3D-SIM Illumination

Laser: 405nm, 488nm, 561nm

3D-SIM emission filters

Bandpass filters: 419-465nm, 500-550nm, 609-654nm

Objective

Plan Apo N 60x/1.42 PSF

Camera

2x sCMOS cameras allowing simultaneous imaging in two colours

Additional devices

Temperature & CO₂ control available for live cell imaging, hardware laser controlled autofocus

Reservation & Training

To schedule training please contact us at microscopy@ imp.ac.at.