M54 - Spinning disk Confocal Olympus (inverted) with incubation and FRAP unit

The M54 Olympus spinning disk confocal is based on an Olympus IX3 Series (IX83) inverted microscope, equipped with a Yokogawa W1 spinning disk (SD) allowing fast confocal acquisition. All components are controlled by CellSense software.
The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. There is also Olympus Z-Drift Compensation (ZDC) hardware autofocus system for long-term stabilization.
In addition to spinning disk acquisition, a FRAP-unit can be used for localized illumination via laser point scanning. This allows for FRAP and photoactivation experiments with fast spinning disk acquisition.

Camera Data Sheet

Conversion Factor Calculation