M54 - Spinning disk Confocal Olympus (inverted) with incubation and FRAP unit

System description

The M54 Olympus spinning disk confocal is based on an Olympus IX3 Series (IX83) inverted microscope, equipped with a Yokogawa W1 spinning disk (SD) allowing fast confocal acquisition. All components are controlled by CellSense software.
The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. There is also Olympus Z-Drift Compensation (ZDC) hardware autofocus system for long-term stabilization.
In addition to spinning disk acquisition, a FRAP-unit can be used for localized illumination via laser point scanning. This allows for FRAP and photoactivation experiments with fast spinning disk acquisition.

Illumination

LED: 400 nm, 465 nm, 565 nm

Transmitted light LED illumination

DIC illumination

Lasers for SD

405

488

561

640

Filters

Filters Vis:

Ex 365/50; Em LP 397; BSP 395
Ex 480/20; Em 530/40; BSP 495
Ex 540/20; Em LP575; BSP 570

Filters SD emission:

447/60
525/50
617/73
685/40

Dichroic for Spinning Disk

405/488/561/640

Objectives

4x / 0.16 WD 13 mm
10x/ 0.4 WD 3.1 mm
20x/ 0.75 WD 0.6 mm
40x / 0.75 WD 0.51 mm
40x / 1.4 (oil) WD 0.13 mm
100x / 1.4 (oil) 0.13 mm

Additional devices

Hardware Autofocus
Incubator and CO2