M52 - STELLARIS 8 CryoCLEM with Falcon FLIM

System description

The Leica STELLARIS 8 FALCON is an upright point laser scanning confocal microscope featuring Fluorescence Lifetime Imaging (FLIM) with FALCON technology for real-time lifetime measurements. The system is equipped with White Light Laser (WWL) which provides flexible excitation across the visible spectrum. M52 has five HyD detectors. The system is equipped with a XY-motorized stage with a Z-Galvo stage insert. The microscope is fully enclosed for heating and with CO2 control. The system can be configured for Cryo-CLEM upon conversion.

Widefield illumination

LED for brightfield

Lasers for confocal imaging

Diode 405 nm

OPSL 488 nm

OBIS 561 nm

Diode 638 nm

White Light Laser (WLL) 440 - 790 nm

Filters

2x HyDS detectors 

2x HyDX detectors (optimized for FLIM)

1x HyDR detector (optimized for FLIM)

T-PMT (transmitted light) detector

Objectives

10X/0.40 DRY HC PL APO CS2

20X/0.75 DRY HC PL APO CS2

25X/0.95 WATER HC FLUOTAR L VISIR

40X/1.25 GLYCEROL HC PL APO CS2

63X/1.40 OIL HC PL APO CS2

 

50X/0.90 CLEM HCX PL APO

Additional devices

Leica K5 camera

Cryo-CLEM equipment