M50 - Spinning Disk Confocal Olympus (inverted) with incubation and FRAP unit

The (M50) Olympus spinning disk confocal is based on an Olympus IX3 Series (IX83) inverted microscope, equipped with a dual-camera Yokogawa W1 spinning disk (SD) allowing fast confocal acquisition. All components are controlled by CellSense software.

The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. There is also Olympus Z-Drift Compensation (ZDC) hardware autofocus system for long-term stabilization. The combination of a sCMOS (ORCA-Fusion) and a EMCCD (Andor) cameras makes it suitable for high resolution, live cell imaging, or experiments requiring high sensitivity of the detector.

In addition to spinning disk acquisition, a FRAP-unit can be used for localized illumination via laser point scanning. This allows for FRAP and photoactivation experiments with fast spinning disk acquisition.

M50 is connected to HIVE