M50 - Spinning Disk Confocal Olympus (inverted) with incubation and FRAP unit

System Description

The (M50) Olympus spinning disk confocal is based on an Olympus IX3 Series (IX83) inverted microscope, equipped with a dual-camera Yokogawa W1 spinning disk (SD) allowing fast confocal acquisition. All components are controlled by CellSense software.

The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. There is also Olympus Z-Drift Compensation (ZDC) hardware autofocus system for long-term stabilization. The combination of a sCMOS (ORCA-Fusion) and a EMCCD (Andor) cameras makes it suitable for high resolution, live cell imaging, or experiments requiring high sensitivity of the detector.

In addition to spinning disk acquisition, a FRAP-unit can be used for localized illumination via laser point scanning. This allows for FRAP and photoactivation experiments with fast spinning disk acquisition.

Illumination

 

LED: 400nm, 465nm, 565nm

Transmitted Light LED illumination

Laser for SD

 

405nm

488nm

561nm

640nm

Filter

 

Filters Vis:

 

Ex 365/50; Em LP 397; BSP 395

Ex 480/20; Em 530/40; BSP 495

Ex 540/20; Em LP575; BSP 570

Ex 631/23; Em 700/50; BSP 660

 

Filters SD-Emission:

 

447/60

525/50

617/73

685/40

Dichroic for Spinning Disk

 

405/488/568/647

Objectives

4x/0.16 (Air) WD 13mm

10x/0.4 (Air) WD 3.1mm

20x/0.75 (Air) WD 0.6mm

40x/0.75 (Air) WD 0.5mm

40x/1.25 (Silicon Oil) WD 0.3mm

100x/1.45 (Oil) WD 0.13mm

 

On request

 

10x/0.3 (Air) WD 10mm

20x/0.5 (Air) WD 2.1mm

30x/1.05 (Silicon Oil) WD 0.8mm

40x/1.4 (Oil) WD 0.18mm

60x/1.42 (Oil) WD 0.15mm

20x/0.7 (Water) WD 0.35mm

60x/1.2 (Water) WD 0.28mm

Cameras

Hamamatsu Orca Fusion CMOS, Pixel Size: 6.5um (2304x2304, 5.3 Megapixels)

https://www.hamamatsu.com/eu/en/product/type/C14440-20UP/index.html

 

Andor Ixon Ultra 888 EMCCD camera, Pixel Size: 13um (1024 x 1024)

http://www.andor.com/cameras/ixon-emccd-camera-series

Additional devices

Additional magnification 3.2x

Motorized XY stage with piezo Z

Hardware Autofocus

Incubator and CO2

Reservation & Training

If you wish to use (or learn more about) the FLIM system, please send an email to microscopy(at)imp.ac.at in order to arrange the training.