M50 - Spinning Disk Confocal Olympus (inverted) with incubation and FRAP unit
The (M50) Olympus spinning disk confocal is based on an Olympus IX3 Series (IX83) inverted microscope, equipped with a dual-camera Yokogawa W1 spinning disk (SD) allowing fast confocal acquisition. All components are controlled by CellSense software.
The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. There is also Olympus Z-Drift Compensation (ZDC) hardware autofocus system for long-term stabilization. The combination of a sCMOS (ORCA-Fusion) and a EMCCD (Andor) cameras makes it suitable for high resolution, live cell imaging, or experiments requiring high sensitivity of the detector.
In addition to spinning disk acquisition, a FRAP-unit can be used for localized illumination via laser point scanning. This allows for FRAP and photoactivation experiments with fast spinning disk acquisition.
LED: 400nm, 465nm, 565nm
Transmitted Light LED illumination
Laser for SD
Ex 365/50; Em LP 397; BSP 395
Ex 480/20; Em 530/40; BSP 495
Ex 540/20; Em LP575; BSP 570
Ex 631/23; Em 700/50; BSP 660
Dichroic for Spinning Disk
4x/0.16 (Air) WD 13mm
10x/0.4 (Air) WD 3.1mm
20x/0.8 (Air) WD 0.6mm
40x/0.75 (Air) WD 0.5mm
40x/1.25 (Silicon Oil) WD 0.3mm
100x/1.45 (Oil) WD 0.13mm
10x/0.3 (Air) WD 10mm
20x/0.5 (Air) WD 2.1mm
30x/1.05 (Silicon Oil) WD 0.8mm
40x/1.4 (Oil) WD 0.18mm
60x/1.42 (Oil) WD 0.15mm
20x/0.7 (Water) WD 0.35mm
60x/1.2 (Water) WD 0.28mm
Hamamatsu Orca Fusion CMOS, Pixel Size: 6.5um (2304x2304, 5.3 Megapixels)
Andor Ixon Ultra 888 EMCCD camera, Pixel Size: 13um (1024 x 1024)
Additional magnification 3.2x
Motorized XY stage with piezo Z
Incubator and CO2
Reservation & Training
If you wish to use (or learn more about) the FLIM system, please send an email to microscopy(at)imp.ac.at in order to arrange the training.