M47 - FLIM - Picoquant Fluorescent Lifetime Imaging Microscope
System Description
The PicoQuant MT200 is an inverted point laser scanning confocal microscope (microscope body Olympus IX83) featuring Fluorescence Lifetime Imaging (FLIM) with Time-Correlated Single Photon Counting (TCSPC) technology. The system is equipped with a multichannel-event-timer (MultiHarp 150, PicoQuant) offering up to 16 independent channels, a galvo scanner (FLIMbee, PicoQuant), and two sensitive PMA hybrid detectors of 50 ps time resolution (PicoQuant). In addition, this system can be used for anisotropy measurements.
The microscope also provides a XY-motorized stage. A large chamber incubator allows both temperature and CO2 control.
Microscope frame
Olympus IX83 inverted microscope frame
Illumination
Sola SM2 (Lumencor)
Excitation
Picosecond LDH pulsed lasers (pulse widths of 20 ps or higher, Sepia PDL 828, PicoQuant):
440 nm
485 nm
510 nm
561 nm
639 nm
Objective lenses (Olympus)
UPlanFLN 10x, NA = 0.3
UPlanSApo 60x, NA = 1.2 (water immersion)
UplanSApo 100x, NA = 1.4 (oil immersion)
On request
20x/0.5 (Air) WD 2.1mm
20x/0.7 (Water) WD 0.35mm
30x/1.05 (Silicon Oil) WD 0.8mm
40x/1.25 (Silicon Oil) WD 0.3mm
40x/1.4 (Oil) WD 0.18mm
60x/1.4 (Oil) WD 0.15mm
Filters for FLIM detectors
475/35nm
514/30nm
525/50nm
535/50nm
620/60nm
645/110nm
650/60nm
690/70nm
Detector
Hybrid Photomultiplier Detection Assembly with < 50 ps time resolution
Analysis Software
SymPhoTime 64 (more details on the Picoquant webpage)