M46 - Elyra7 with lattice SIM2

System Description

The M46 system is a super-resolution systemIt features 3D single molecule localisation SMLM, lattice structure illumination SIM2, SIM2 Apotome, TIRF 

SMLM with techniques like PALM, dSTORM or PAINT bring lateral resolution down to 20-30nm. The reshaping of PSF brings the axial resolution down to 50-80nm across 1.4um volume 

The lattice SIM pattern allows fast acquisition compared to a classical SIM and the SIM2 reconstruction achieves XY resolution down to 60nm and can be used with standard fluorescent slides and probes. 

Dual camera link and unique image processing algorithms allow fast (simultaneous) two-colour imaging (up to 255fps)

Widefield Illumination

Sola SM - Fluorescence 

LED for brightfield 

Laser for Imaging

405nm 
488nm 
561nm 
642nm 

Filters

Dual Camera Link 

SBS LP560 to Camera1 
SBS BP 490-560 + LP640 to Camera1 
Mirror to Camera2 
SBS LP660 to Camera1

Objectives

10x/0.3 EC plan-neofluar DIC 
20x/0.8 plan-apochromat Var3 
63x/1.4 plan-apochromat Oil DIC 
63x/1.46 alpha plan-apochromat Oil Var2

Camera

Pixel Size: 6.5μm