M44 - Spinning Disk Confocal Olympus (inverted) with incubation/Superresolution (SoRa)

System Description

The M44 Olympus spinning disk confocal is based on an Olympus IX3 Series (IX83) inverted microscope, equipped with a Yokogawa W1 spinning disk (SD). All components are controlled by CellSense software.

The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, Z-stacks, time-lapse. There is also a Olympus Z-Drift Compensation (ZDC) hardware autofocus system for long-term stabilization.

This makes it suitable for various live experiments or the automatic acquisition of a large amount of images (screening).

Additionally, an extra disk is installed to deliver images with up to 2-times enhanced spatial resolution, using super-resolution optical photon reassignment (SoRa) technology.

Illumination

LED: 400nm, 465nm, 565nm

Transmitted Light LED illumination

 

 

Lasers for SD

405nm

488nm

514nm

561nm

640nm

 

 

Filters

Filters Vis:

Ex 365/50; Em LP 397; BSP 395

Ex 480/20; Em 530/40; BSP 495

Ex 540/20; Em LP575; BSP 570


Filters SD-Emission:

447/60

525/50

578/105

617/73

685/40

 

 

Dichroic for spinning disk

445/514/640

405/488/568/647

Objectives

4x/0.16 (Air) WD 13mm

10x/0.4 (Air) WD 3.1mm

20x/0.75 (Air) WD 0.6mm

40x/0.75 (Air) WD 0.5mm

40x/1.25 (Silicon Oil) WD 0.3mm

100x/1.45 (Oil) WD 0.13mm

 

On request

4x/0.16 (Air) WD 13mm

10x/0.3 (Air) WD 10mm

20x/0.5 (Air) WD 2.1mm

30x/1.05 (Silicon Oil) WD 0.8mm

40x/1.4 (Oil) WD 0.18mm

60x/1.42 (Oil) WD 0.15mm

20x/0.7 (Water) WD 0.35mm

60x/1.2 (Water) WD 0.28mm

 

 

Additional devices

Motorized XY stage with piezo Z

Hardware Autofocus

Incubatior and CO2

Super-resolution spinning-disk confocal microscopy using optical photon reassignment