M43 - Pannoramic FLASH 250 III
The M43 system is an automated, extremely fast (2x faster than M28 in bright field), widefield microscope equipped with transmitted and fluorescent light source. It can be used to scan up to 300 slides in both transmitted and fluorescence. This system comes with darkfield preview mode which is used to recognize samples to be scanned in the fluorescence as well as in the transmitted light mode.
The advantages of this system are the constant illumination and white balance as well as the elimination of the operator induced error. The system can acquire Z-stacks and deliver maximum intensity projection (MIP) important for 3D structures. As a result, you get images of the whole sample (and not only of the preferentially selected area) that can be subjected to automated image analysis.
The system can run on its own, no attendance needed. Slides for the automatic scanner have to be prepared in a specific way. Please ask the BioOptics staff for directions or refer to Protocols.
This system is exclusively operated by the BioOptics facility staff. It helps to automate your experiments and fits into the chain of - automated staining - imaging - image analysis.
- Arrangement for scanning MUST be done with the Facility Staff - please book via JIRA Service Desk
- Embedding medium for the slides to be scanned MUST be dried (see protocol) – don’t put nail polish around the coverslip
Last minute scans will not always be possible, so please plan in advance!
Lumencor Spectra 6 – Solid state light engine
WhiteLight LED for transmitted light
Ex 365/18nm Em 397/18nm
Ex 485/26nm Em 521/27nm
Ex 559/32nm Em 607/40nm
Ex 649/19nm Em 700/57nm
Ex 480/40nm Em 535/50nm
Ex 560/55nm Em 645/75nm
Ex 620/60nm Em 700/75nm
Adimec Quartz Q12A180 – transmitted light – pixel size on the image 0,242797µm (20x objective) and 0,1214µm (40x objective) PCO.edge – fluorescence – pixel size on the image – 0,3250µm (only 20x objective)