
M41 – LSM980 Axio Observer (inverted) with Airyscan II and incubation
System Description

The M41 system is an inverted point laser scanning confocal microscope – LSM980. It is a spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. LSM980 features 4 highly sensitive GaAsP (Gallium Arsenide) detectors with a QE of 45% and up to 2x SNR as compared to standards PMTs.
The system is equipped with a motorized xyz-stage and a definite focus (hardware autofocus device). The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.
In addition the M41 system features Airyscan II - a hexagonal GaAsP detector. This device enables improvement of the resolving power in XYZ by a factor of 1.7. The fast mode of the AiryScan called AiryScan MPLX allows 4-8x faster acquisition with up to 1.5x improvement of the optical resolving power and up to 4x increase of signal-to-noise ratio (SNR) compared to 1AU confocal mode.
This microscope is controlled with the ZEN Blue software.
Widefield Illumination
LED
LED for brightfield
Lasers for Confocal Imaging
405nm
445nm
488nm
514nm
561nm
639nm
Filter
CH1: 380-736
CH2: 380-736
CHS1-4 GaAsP: 409-694
CHD: T-PMT (transmitted light)
Splitter to AiryScan Detector: SP 505; LP 525; LP570; SP 550; LP460; SP 615; Plate; Mirror
AIRYSCAN: BP 420-480+BP495-550; BP 420-480+BP570-630; BP 420-480+LP605; BP 465-505+BP525-585; BP 495-550+BP570-630; BP 495-555+LP660; BP 570-620+LP 660
Objectives
2.5x/0.085 plan-neofluar 10x/0.45 plan-apochromat 20x/0.8 plan-apochromat 40x/1.1 LD plan-apochromat Water 40x/1.3 EC plan-neofluar 63x/1.4 plan-apochromat Oil DIC
Additional Devices
Incubation with CO2
XY Motorized Stage
Piezo Z stage insert
Multiple Position Acquisition
High Dynamic Range acquisition (HDR)
FRAP
Tiles