M40 - Spinning Disk Confocal Olympus (inverted) with incubation
System Description
The M40 Olympus spinning disk confocal is based on an Olympus IX3 Series (IX83) inverted microscope, equipped with a dual-camera Yokogawa W1 spinning disk (SD) allowing fast confocal acquisition. All components are controlled by the CellSense software.
The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. There is also Olympus Z-Drift Compensation (ZDC) hardware autofocus system for long-term stabilization.
This makes it suitable e.g. for various live experiments or the automatic acquisition of a large amount of images (screening).
Illumination
LED: 400nm, 465nm, 565nm
Transmitted Light LED illumination
Lasers for SD
405nm
488nm
561nm
640nm
Filters
Filters Vis:
Ex 365/50; Em LP 397; BSP 395
Ex 480/20; Em 530/40; BSP 495
Ex 540/20; Em LP575; BSP 570
Ex 631/23; Em 700/50; BSP 660
Filters SD-Emission:
Camera 1:
447/60
525/50
617/73
685/40
Camera 2:
447/60
525/50
Dichroic for spinning disk
405/488/568/647
Objectives
4x/0.16 UPLSApo WD -13mm
10x/0.4 UPLSApo WD – 3.1mm
10x/0.3 UPLFApo WD –10mm
20x/0.75 UPLSApo WD – 0.6mm
40x/0.75 UPLFLN WD – 0.5mm
40x/1.25 Silicon Oil UPLSApo WD – 0.3mm
On request
30x/1.05 Silicon Oil UPLSApo WD – 0.8mm
40x/1.4 Oil UPLXApo WD – 0.18mm
60x/1.42 Oil UPLXApo WD – 0.15mm
100x/1.45 Oil UPLXApo WD – 0.13mm
Camera
Camera 1 and 2 – Hamamatsu Orca Flash 4.0, Pixel Size: 6.5μm
Additional magnification 3.2x
Additional devices
Motorized XY stage with piezo Z
Hardware Autofocus
Incubatior and CO2