M40 - Spinning Disk Confocal Olympus (inverted) with incubation
System Description
The M40 Olympus spinning disk confocal is based on an Olympus IX3 Series (IX83) inverted microscope, equipped with a dual-camera Yokogawa W1 spinning disk (SD) allowing fast confocal acquisition. All components are controlled by the CellSense software. ScanR software is also available.
The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. There is also Olympus Z-Drift Compensation (ZDC) hardware autofocus system for long-term stabilization.
This makes it suitable e.g. for various live experiments or the automatic acquisition of a large amount of images (screening).
M40 is connected to HIVE
Illumination
LED: 400nm, 465nm, 565nm
Transmitted Light LED illumination
Lasers for SD
405nm
488nm
561nm
640nm
Filters
Filters Vis:
Ex 365/50; Em LP 397; BSP 395
Ex 480/20; Em 530/40; BSP 495
Ex 540/20; Em LP575; BSP 570
Ex 631/23; Em 700/50; BSP 660
Filters SD-Emission:
Camera 1:
447/60
525/50
617/73
685/40
Camera 2:
447/60
525/50
Dichroic for spinning disk
405/488/568/647
Objectives
4x/0.16 (Air) WD 13mm
10x/0.4 (Air) WD 3.1mm
20x/0.75 (Air) WD 0.6mm
40x/0.75 (Air) WD 0.5mm
60x/1.42 (Oil) WD 0.15mm
100x/1.46 (Oil) WD 0.13mm
On request
10x/0.3 (Air) WD 10mm
20x/0.5 (Air) WD 2.1mm
20x/0.7 (Water) WD 0.35mm
30x/1.05 (Silicon Oil) WD 0.8mm
40x/1.25 (Silicon Oil) WD 0.3mm
40x/1.4 (Oil) WD 0.18mm
60x/1.2 (Water) WD 0.28mm
60x/1.4 (Oil) WD 0.15mm
Camera
Camera 1 and 2 – Hamamatsu Orca Flash 4.0, Pixel Size: 6.5μm
Additional magnification 3.2x
Additional devices
Motorized XY stage with piezo Z
Hardware Autofocus
Incubator and CO2