M40 - Spinning Disk Confocal Olympus (inverted) with incubation

System Description

The M40 Olympus spinning disk confocal is based on an Olympus IX3 Series (IX83) inverted microscope, equipped with a dual-camera Yokogawa W1 spinning disk (SD) allowing fast confocal acquisition. All components are controlled by the CellSense software.

The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. There is also Olympus Z-Drift Compensation (ZDC) hardware autofocus system for long-term stabilization.

This makes it suitable e.g. for various live experiments or the automatic acquisition of a large amount of images (screening).

Illumination

LED: 400nm, 465nm, 565nm

Transmitted Light LED illumination

Lasers for SD

405nm

488nm

561nm

640nm

Filters

Filters Vis:

Ex 365/50; Em LP 397; BSP 395

Ex 480/20; Em 530/40; BSP 495

Ex 540/20; Em LP575; BSP 570

Ex 631/23; Em 700/50; BSP 660


Filters SD-Emission:

 

Camera 1:

447/60

525/50

617/73

685/40

 

Camera 2:

447/60

525/50

Dichroic for spinning disk

405/488/568/647

Objectives

10x/0.3 (Air) WD 10mm

10x/0.4 (Air) WD 3.1mm

20x/0.75 (Air) WD 0.6mm

40x/0.75 (Air) WD 0.5mm

40x/1.25 (Silicon Oil) WD 0.3mm

100x/1.45 (Oil) WD 0.13mm

 

On request

4x/0.16 (Air) WD 13mm

10x/0.3 (Air) WD 10mm

20x/0.5 (Air) WD 2.1mm

30x/1.05 (Silicon Oil) WD 0.8mm

40x/1.4 (Oil) WD 0.18mm

60x/1.42 (Oil) WD 0.15mm

20x/0.7 (Water) WD 0.35mm

60x/1.2 (Water) WD 0.28mm

Camera

Camera 1 and 2 – Hamamatsu Orca Flash 4.0, Pixel Size: 6.5μm

Additional magnification 3.2x

Additional devices

Motorized XY stage with piezo Z

Hardware Autofocus

Incubator and CO2