M34 - Discoverer 7 fully automated live cell imaging system
System Description
The M34 - CellDiscoverer 7 is a widefield microscope for brightfield and fluorescence. It is equipped with temperature (heating and cooling), CO2 and humidity control, a motorized XYZ-stage as well as a CMOS and CCD cameras. All components are controlled by ZEN Blue. The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. The combination of a wide range of magnifications (0.25x-100x) with different plate or dish formats makes it suitable e.g. for various live cell experiments or the automatic acquisition of many images (screening).
New optical design and very stable environmental control make this microscope best suitable for long term imaging experiments.
Illumination
Fluorescence LED - 385 / 420 / 470 / 520 / 567 / 590 / 625 nm
Infrared (IR) transmitted light contrast
Phase gradient-contrast for brightfield images of non-stained samples
Filter
Filter system for e.g. CFP-YFP-mCherry-SiRhodamine
EX: 385nm, 470nm, 555nm and 625nm
BS: 405+493+575+653
EM: 425/30+514/30+592/25+709/100
Filter system for e.g. Hoechst/DAPI-YFP-mCherry
EX: 420nm, 520nm and 590nm
BS: 450+538+610
EM: 467/24+555/25+687/145
Filter system for e.g. BFP-GFP-mCherry
EX: 385nm, 470nm and 590nm
BS: 405+493+610
EM: 425/30+524/50+688/145
Filter system for e.g. GFP-mCherry and transmitted light
EX: 470nm and 555nm
BS: 493+575
EM: 514/32+605/50+730/60 (transmitted light)
Filter system for e.g. BFP-YFP and transmitted light
EX: 385nm and 520nm
BS: 405+538
EM: 444/69+581/77+730/60 (transmitted light)
Objectives
Plan-Apochromat 5x/0,35
(in combination with magnification changer 0,5x – 0.25x/0.2)
Plan-Apochromat 20x/0,7
autocorr - (in combination with magnification changer 0,5x / 1x / 2x-10x /0,35; 20x /0,7, 40x/0,7)
Plan-Apochromat 20x/0,95
autocorr - (in combination with magnification changer 0,5x / 1x / 2x - 10x /0,5, 20x /0,8, 40x /0,95)
Plan-Apochromat 50x/1,2 W
autocorr mit Autoimmersion - (in combination with magnification changer 0,5x / 1x / 2x- 25x /1,2 W, 50x / 1,2 W, 100x / 1,2 W)
Camera
Axiocam 506 -CCD (Pixel size: 4.54µm) and Hamamatsu Orca Flash 4.0 (Pixel Size: 6.5μm)
Additional Devices
Incubation chamber with temperature control – heating and cooling
Motorized XY stage with piezo Z - suitable for various dishes, multiwell plates, slides (contact BioOptics to check which multiwell plates are best suited for the Discoverer).
Pipetting port allowing addition of liquids to plates
UV disinfection insert
Specimen Inserts
Multiwell plates (contact BioOptics to check which multiwell plates are best suited for Discoverer)
Insert for 6 Petri dishes 34-36mm
Insert for 1 Petri dish 30-38mm or 50-60mm
Insert for 2 slides 76x26 mm
Insert for 2 slides/Lab-TekTM Chambers 57x26 mm