M33 - LSM 880 Axio Observer (inverted) with Airyscan /Airyscan fast and incubation and 40x/1.2 W, Glycerol AutoCorr objective
The M33 system is an inverted point laser scanning confocal microscope (LSM880).It is a complete spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. LSM880 features a highly sensitive array of 32 GaAsP (Gallium Arsenide) detectors with a QE of 45% and up to 2x SNR as compared to standards PMTs.
The system is equipped with a motorized xyz-stage and a definite focus (hardware autofocus device). The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.
In addition the M33 system features Airyscan - a hexagonal GaSaP detector. This device enables improvement of the resolving power in XYZ by a factor of 1.7. The Airyscan fast option allows 4x faster acquisition with up to 1.5x improvement of the optical resolving power and 4x increase of signal-to-noise ratio (SNR) compared to 1AU confocal mode.
LED for brightfield
Lasers for Confocal Imaging
Laser Diode 405 25mW
Argon 458, 488, 514 30mW
DPSS 561 15mW
HeNe 594 2mW
HeNe 633 5mW
CH3 Quasar (GaAsP) : 416-696 in 8.3nm steps
CHD: T-PMT (transmitted light)
AIRYSCAN: BP 420-480+BP495-550; BP 420-480+BP495-620; BP 420-480+LP605; BP 465-505+LP525; BP 495-550+LP570; BP 570-620+LP645
40x/1.3 EC plan-neofluar Oil DIC
LD LCI 40x/1.2 Water, Glycerol DIC AutoCorr
63x/1.4 plan-apochromat Oil DIC
Incubation with CO2
XY Motorized Stage
Piezo Z stage insert
Multiple Position Acquisition
High Dynamic Range acquisition (HDR)