M33 - LSM 880 Axio Observer (inverted) with Airyscan /Airyscan fast and incubation and 40x/1.2 W, Glycerol AutoCorr objective

The M33 system is an inverted point laser scanning confocal microscope (LSM880).It is a complete spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. LSM880 features a highly sensitive array of 32 GaAsP (Gallium Arsenide) detectors with a QE of 45% and up to 2x SNR as compared to standards PMTs.

The system is equipped with a motorized xyz-stage and a definite focus (hardware autofocus device). The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.

In addition the M33 system features Airyscan - a hexagonal GaSaP detector. This device enables improvement of the resolving power in XYZ by a factor of 1.7. The Airyscan fast option allows 4x faster acquisition with up to 1.5x improvement of the optical resolving power and 4x increase of signal-to-noise ratio (SNR) compared to 1AU confocal mode.

M33 is connected to HIVE