
M20 - TIRF 3 System Axio Observer (inverted) with incubation
System Description

The M20 system is an inverted widefield microscope for brightfield and fluorescence. It is equipped with temperature, CO2 and humidity control, a motorized xyz-stage, an EMCCD camera and in addition with a motorized TIRF (Total Internal Reflection Fluorescence) insert allowing high resolution visualization of structures within 100nm of the coverslip. All components are controlled by Axiovision. The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. Using the TIRF insert is especially useful for visualization of membrane bound components.
Illumination
Short Arc Lamp 120W
Halogen Lamp 50W
Lasers for TIRF
405nm 50mW
488nm 100mW
561nm 40mW
639nm 30mW
Filters
Ex 488/10nm | Em 525/50nm |
Ex 406+493+561nm | Em 460+525+608nm |
Ex 483+564+642nm | Em 526+601+688nm |
Em 525/50nm | |
Em 600/50nm | |
Em 692/10nm | |
Em 700/75nm |
Objectives
63x/1.4 plan-apochromat Oil DIC III
100x/1.46 alpha plan-apochromat Oil DIC III
Additional Devices
Incubation chamber with temperature and CO2 control
motorized XY stage