M01 - Spinning Disk Confocal Axio Observer (inverted)

System Description

The M01 system is an inverted microscope equipped with a Yokogawa CSU X1 spinning disc (SD) allowing fast confocal acquisition. All components are controlled by the ZEN blue software.
The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. There is also a ZEISS Definite Focus 2 hardware autofocus system for long-term stabilization.
This makes it suitable e.g. for various live experiments (caution: the system does not have an incubator) or the automatic acquisition of a large amount of images (screening).






LED: 400nm, 465nm, 565nm
Halogen Lamp 50W

Laser for SD



Filters Vis:

Ex 365/12; Em LP 397; BSP 395
Ex 470/40; Em 525/50; BSP 495
Ex 550/25; Em 605/70; BSP 570

Filters SD-Emission:

450/50nm 525/50nm 562/45nm 629/62nm 690/50nm

Dichroics for Spinning Disc



10x/0.3 EC plan Neofluar Ph1 DICI
20x/0.8 plan-Apochromat
32x/0.85 C-Achroplan W Korr
10x/0.45 C-Apochromat Water
63x/1.4 plan-apochromat Oil DICIII
100x/1.46 alpha-plan-fluar Oil DICIII






SD: Hamamatsu EMCCD 9100-13 – 16µm pixel with 1.2x additional magnification

Hamamatsu Orca Flash 4.0, Pixel Size: 6,5µm

Additional devices

Motorized XY ASI stage with piezo Z.
Definite Focus