M01 - Spinning Disk Confocal Axio Observer (inverted)
The M01 system is an inverted microscope equipped with a Yokogawa CSU X1 spinning disc (SD) allowing fast confocal acquisition. All components are controlled by the ZEN blue software.
The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. There is also a ZEISS Definite Focus 2 hardware autofocus system for long-term stabilization.
This makes it suitable e.g. for various live experiments (caution: the system does not have an incubator) or the automatic acquisition of a large amount of images (screening).
LED: 400nm, 465nm, 565nm
Halogen Lamp 50W
Laser for SD
Ex 365/12; Em LP 397; BSP 395
Ex 470/40; Em 525/50; BSP 495
Ex 550/25; Em 605/70; BSP 570
450/50nm 525/50nm 562/45nm 629/62nm 690/50nm
Dichroics for Spinning Disc
10x/0.3 EC plan Neofluar Ph1 DICI
32x/0.85 C-Achroplan W Korr
10x/0.45 C-Apochromat Water
63x/1.4 plan-apochromat Oil DICIII
100x/1.46 alpha-plan-fluar Oil DICIII
SD: Hamamatsu EMCCD 9100-13 – 16µm pixel with 1.2x additional magnification
Hamamatsu Orca Flash 4.0, Pixel Size: 6,5µm
Motorized XY ASI stage with piezo Z.