F14 - Penteon
System Description
This analyzer from Novocyte provides the flexibility to choose from up to 30 fluorescence channels utilizing up to 5 lasers. The NovoExpress software provides functionality in data acquisition, analysis and reporting. The 7-log dynamic range eliminates the need for routine detector adjustments, which means that samples can be acquired right away, saving on set up time for experiments and eliminating the risk for incorrect signal amplification settings. Sample delivery is automated and made convenient through use of an auto-loader, which supports up to 40 5ml tubes and 24-, 48-, 96-, and 384-well plates. A volume measurement enables the calculation of cell concentration for each sample.
Laser 1
349nm 20mW Ultraviolet Laser
Detection
UV445 445/45 nm - DAPI, Hoechst 33258, BUV395
UV525 525/45 nm - BUV496
UV586 586/20 nm - BUV563
UV615 615/20 nm - BUV615
UV667 667/30 nm - BUV661
UV725 725/40 nm - BUV737
UV780 780/60 nm - BUV805
Laser 2
405nm 100mW Violet Laser
Detection
V445 445/45 nm - BV421, BFP, AF405
V525 525/45 nm - BV510
V586 586/20 nm - BV570
V615 615/20 nm - BV605
V667 667/30 nm - BV650
V725 725/40 nm - BV711
V780 780/60 nm - BV785
Laser 3
488nm 100mW Blue Laser
Detection
B525 525/45 nm - FITC, GFP, AF488, RB545, Venus green, YFP
B586 586/20 nm -
B615 615/20 nm - RB613
B667 667/30 nm - RB670
B695 695/40 nm - PerCP-Cy5.5, RB705
B725 725/40 nm - RB744
Laser 4
561nm 100mW YellowGreen Laser
Detection
Y586 586/20 nm - PE, tdtomato, DsRed, Atto550
Y615 615/20 nm - mcherry, PE-TexasRed, PI
Y667 667/30 nm - 7-AAD, PE-Cy5
Y695 695/40 nm - PE-Cy5.5
Y725 725/40 nm -
Y780 780/60 nm - PE-Cy7
Laser 5
637nm 100mW Red laser
Detection
R667 667/30 nm - APC, AF647, AF660
R695 695/40 nm -
R725 725/40 nm - AF700
R780 780/60 nm - APC-Cy7