F05 - FACS Aria IIu
System Description
The FACS Aria IIu is a 3 laser sorter with 10 fluorescence detectors controlled by FACSDiva software and operated exclusively by BioOptics staff members. It allows bulk sorts of up to 4 populations simultaneously. Temperature control for sample input and sort output (4oC to room temperature) is possible. The nozzle sizes 70μm - 85μm - 100μm - 130μm are availabe.
Fluorochromes in bold letters are listed in the configurations of the flow cytometer.
Laser 1
405 nm Violet Laser, 50mW
Detection
450/40 nm - AF 405, BFP, BV 421, Cascade Blue, DAPI, Hoechst 33342, Pacific Blue
530/30 nm - AF 430, AmCyan, Ametrine, BD Horizon V500, BV 510, QDot 565
785/60 nm - BV 786, QDot 800, (BV570 in Configuration)
Laser 2
488 nm Blue Laser, Coherent Sapphire 20mW
Detection
488/10 nm - SSC
530/30 nm - AF 488, FITC, GFP, Venus, YFP
575/26 nm - PE
610/20 nm - PE-Texas Red, PI
675/20 nm - 7-AAD, CyChrome, PE-Cy5, PerCP, PerCP-Cy5.5
780/60 nm - PE-Cy7
Laser 3
633 nm Red Laser, HeNe 17mW
Detection
675/20 nm - AF 647, AF 660, APC
780/60 nm - APC-Cy7, APC-H7