We inject your DNA or BAC solution to the pronucleus of zygotes.
Typically we inject DNA solutions at a concentration of ~3 ng/µl, BAC solutions at ~1 ng/µl.
Your stocksolution should have a concentration of 50-100 ng/µl, solved in microinjection buffer ( 5mM Tris, 0.1 mM EDTA in ultrapure water, pH 7.4, 0.22 µm filtered). We dilute it accordingly.
For BAC DNA, additionally a polyamine mix (spermin, spermidine) and NaCl is added.
CRISPR/Cas9 mixes are injected either into the pronucleus or the cytoplasma of zygotes.
Please submit the Injection Request form "Inj request_DNA_BAC" or "Inj request_CRISPR".