Cryopreservation of Mouse strains by sperm freezing and embryo freezing



  • Our basic strategy is to freeze SPERM of each mouse line.
  • We test fertility by thawing sperm and perform a test-IVF (in-vitro fertilization, and culture to the 2-cell stage), at least for strains generated in-house.
  • Additionally, we freeze embryos (2-cell stage) for strains that were generated in-house and and are homozygous or compound 


What do we need for sperm freezing?


We need 2 males (and maybe a third as backup), best between 3-8 month old, and proven breeders are preferred.

We need information about the background strain and the mutations (kind, genotype).

If you know the MGI-ID (Mouse Genome Informatics) of the mutation(s) it would also help a lot.

We freeze ~20 semen straws for each strain.


What do we need for embryo freezing?


Depending on their age we need 4-8 females to freeze homozygous embryos. Females at 3-4 weeks old respond best to hormones.

We use IVF (in-vitro fertilization) to produce embryos for freezing. We use frozen/thawed sperm for the IVF. By this we can also see if the sperm survived freezing.


Depending on the strain and background the survival rate of frozen/thawed IVF-embryos can vary to a large degree. Therefore we cannot guarantee that we can rederivate embryos from each single strain we freeze.



Since 2017 we use media from CosmoBio for freezing (FERTIUP®) and IVF(CARD®).