Publications

2017

Root diffusion barrier control by a vasculature-derived peptide binding to the SGN3 receptor.
Doblas VG, Smakowska-Luzan E, Fujita S, Alassimone J, Barberon M, Madalinski M, Belkhadir Y, Geldner N.
Science. 2017 Jan 20;355(6322):280-284. doi: 10.1126/science.aaj1562. (abstract)

2016

SIRT2 regulates nuclear envelope reassembly through ANKLE2 deacetylation.
Kaufmann T, Kukolj E, Brachner A, Beltzung E, Bruno M, Kostrhon S, Opravil S, Hudecz O, Mechtler K, Warren G, Slade D.
J Cell Sci. 2016 Dec 15;129(24):4607-4621. (abstract)

Arginine phosphorylation marks proteins for degradation by a Clp protease.
Trentini DB, Suskiewicz MJ, Heuck A, Kurzbauer R, Deszcz L, Mechtler K, Clausen T.
Nature. 2016 Nov 3;539(7627):48-53. doi: 10.1038/nature20122. (abstract)

Linear ubiquitination by LUBEL has a role in Drosophila heat stress response.
Asaoka T, Almagro J, Ehrhardt C, Tsai I, Schleiffer A, Deszcz L, Junttila S, Ringrose L, Mechtler K, Kavirayani A, Gyenesei A, Hofmann K, Duchek P, Rittinger K, Ikeda F.
EMBO Rep. 2016 Nov;17(11):1624-1640. (abstract)

Molecular basis for cytoplasmic RNA surveillance by uridylation-triggered decay in Drosophila.
Reimão-Pinto MM, Manzenreither RA, Burkard TR, Sledz P, Jinek M, Mechtler K, Ameres SL.
EMBO J. 2016 Nov 15;35(22):2417-2434. (abstract)

CCAN Assembly Configures Composite Binding Interfaces to Promote Cross-Linking of Ndc80 Complexes at the Kinetochore.
Pekgöz Altunkaya G, Malvezzi F, Demianova Z, Zimniak T, Litos G, Weissmann F, Mechtler K, Herzog F, Westermann S.
Curr Biol. 2016 Sep 12;26(17):2370-8. doi: 10.1016/j.cub.2016.07.005. (abstract)

A Combination of CRISPR/Cas9 and Standardized RNAi as a Versatile Platform for the Characterization of Gene Function.
Wissel S, Kieser A, Yasugi T, Duchek P, Roitinger E, Gokcezade J, Steinmann V, Gaul U, Mechtler K, Förstemann K, Knoblich JA, Neumüller RA.
G3 (Bethesda). 2016 Aug 9;6(8):2467-78. doi: 10.1534/g3.116.028571. (abstract)

Mechanism of APC/CCDC20 activation by mitotic phosphorylation.
Qiao R, Weissmann F, Yamaguchi M, Brown NG, VanderLinden R, Imre R, Jarvis MA, Brunner MR, Davidson IF, Litos G, Haselbach D, Mechtler K, Stark H, Schulman BA, Peters JM.
Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2570-8. doi: 10.1073/pnas.1604929113 (abstract)

Polysialylation controls dendritic cell trafficking by regulating chemokine recognition.
Kiermaier E, Moussion C, Veldkamp CT, Gerardy-Schahn R, de Vries I, Williams LG, Chaffee GR, Phillips AJ, Freiberger F, Imre R, Taleski D, Payne RJ, Braun A, Förster R, Mechtler K, Mühlenhoff M, Volkman BF, Sixt M.
Science. 2016 Jan 8;351(6269):186-90. doi: 10.1126/science.aad0512. (abstract)

2015

Comprehensive Cross-Linking Mass Spectrometry Reveals Parallel Orientation and Flexible Conformations of Plant HOP2-MND1.
Rampler E, Stranzl T, Orban-Nemeth Z, Hollenstein DM, Hudecz O, Schloegelhofer P, Mechtler K.
J Proteome Res. 2015 Dec 4;14(12):5048-62. doi: 10.1021/acs.jproteome.5b00903. (abstract)

Arabidopsis PCH2 Mediates Meiotic Chromosome Remodeling and Maturation of Crossovers.
Lambing C, Osman K, Nuntasoontorn K, West A, Higgins JD, Copenhaver GP, Yang J, Armstrong SJ, Mechtler K, Roitinger E, Franklin FC.
PLoS Genet. 2015 Jul 16;11(7):e1005372. doi: 10.1371/journal.pgen.1005372. eCollection 2015 Jul. (abstract)

Casein Kinase 1 and Phosphorylation of Cohesin Subunit Rec11 (SA3) Promote Meiotic Recombination through Linear Element Formation.
Phadnis N, Cipak L, Polakova S, Hyppa RW, Cipakova I, Anrather D, Karvaiova L, Mechtler K, Smith GR, Gregan J.
PLoS Genet. 2015 May 20;11(5):e1005225. doi: 10.1371/journal.pgen.1005225. eCollection 2015 May. (abstract)

Rio1 promotes rDNA stability and downregulates RNA polymerase I to ensure rDNA segregation.
Iacovella MG, Golfieri C, Massari LF, Busnelli S, Pagliuca C, Dal Maschio M, Infantino V, Visintin R, Mechtler K, Ferreira-Cerca S, De Wulf P.
Nat Commun. 2015 Apr 8;6:6643. doi: 10.1038/ncomms7643. (abstract)

Anion-exchange chromatography of phosphopeptides: weak anion exchange versus strong anion exchange and anion-exchange chromatography versus electrostatic repulsion-hydrophilic interaction chromatography.
Alpert AJ, Hudecz O, Mechtler K.
Anal Chem. 2015 May 5;87(9):4704-11. doi: 10.1021/ac504420c. Epub 2015 Apr 17. (abstract)

Quantitative phosphoproteomics of the ataxia telangiectasia-mutated (ATM) and ataxia telangiectasia-mutated and rad3-related (ATR) dependent DNA damage response in Arabidopsis thaliana.
Roitinger E, Hofer M, Köcher T, Pichler P, Novatchkova M, Yang J, Schlögelhofer P, Mechtler K.
Mol Cell Proteomics. 2015 Mar;14(3):556-71. doi: 10.1074/mcp.M114.040352. Epub 2015 Jan 5. (abstract)

To top

2014

MS Amanda, a universal identification algorithm optimized for high accuracy tandem mass spectra.
Dorfer, V., Pichler, P., Stranzl, T., Stadlmann, J., Taus, T., Winkler, S., Mechtler, K. (2014).  J Proteome Res. 13(8):3679-84 (abstract)

Global analysis of muscle-specific kinase signaling by quantitative phosphoproteomics.
Dürnberger, G., Camurdanoglu, BZ., Tomschik, M., Schutzbier, M., Roitinger, E., Hudecz, O., Mechtler, K., Herbst, R. (2014).  Mol Cell Proteomics. 13(8):1993-2003 (abstract)

Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids.
Hofbauer, HF., Schopf, FH., Schleifer, H., Knittelfelder, OL., Pieber, B., Rechberger, GN., Wolinski, H., Gaspar, ML., Kappe, CO., Stadlmann, J., Mechtler, K., Zenz, A., Lohner, K., Tehlivets, O., Henry, SA., Kohlwein, SD. (2014). . Dev Cell. 29(6):729-39 (abstract)

A cooperative mechanism drives budding yeast kinetochore assembly downstream of CENP-A.
Hornung, P., Troc, P., Malvezzi, F., Maier, M., Demianova, Z., Zimniak, T., Litos, G., Lampert, F., Schleiffer, A., Brunner, M., Mechtler, K., Herzog, F., Marlovits, TC., Westermann, S. (2014).
J Cell Biol. 206(4):509-24 (abstract)

Development and performance evaluation of an ultralow flow nanoliquid chromatography-tandem mass spectrometry set-up.
Köcher, T., Pichler, P., De Pra, M., Rieux, L., Swart, R., Mechtler, K. (2014).
Proteomics. 14(17-18):1999-2007 (abstract)

The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes.
Mérai, Z., Chumak, N., García-Aguilar, M., Hsieh, TF., Nishimura, T., Schoft, VK., Bindics, J., Slusarz, L., Arnoux, S., Opravil, S., Mechtler, K., Zilberman, D., Fischer, RL., Tamaru, H. (2014).  Proc Natl Acad Sci U S A. 111(45):16166-71 (abstract)

Deep and precise quantification of the mouse synaptosomal proteome reveals substantial remodeling during postnatal maturation.
Moczulska, KE., Pichler, P., Schutzbier, M., Schleiffer, A., Rumpel, S., Mechtler, K. (2014).  J Proteome Res. 13(10):4310-24 (abstract)

3DSpectra: A 3-dimensional quantification algorithm for LC-MS labeled profile data.
Nasso, S., Hartler, J., Trajanoski, Z., Di Camillo, B., Mechtler, K., Toffolo, GM. (2014).  J Proteomics. 112C:156-165 (abstract)

H3S28 phosphorylation is a hallmark of the transcriptional response to cellular stress.
Sawicka, A., Hartl, D., Goiser, M., Pusch, O., Stocsits, RR., Tamir, IM., Mechtler, K., Seiser, C. (2014).  Genome Res. 24(11):1808-20 (abstract)

Quantitative phosphoproteomics reveals the role of protein arginine phosphorylation in the bacterial stress response.
Schmidt, A., Trentini, DB., Spiess, S., Fuhrmann, J., Ammerer, G., Mechtler, K., Clausen, T. (2014).  Mol Cell Proteomics. 13(2):537-50 (abstract)

Chasing phosphoarginine proteins: development of a selective enrichment method using a phosphatase trap.
Trentini, DB., Fuhrmann, J., Mechtler, K., Clausen, T. (2014).  Mol Cell Proteomics. (abstract)

A DEK Domain-Containing Protein Modulates Chromatin Structure and Function in Arabidopsis.
Waidmann, S., Kusenda, B., Mayerhofer, J., Mechtler, K., Jonak, C. (2014).  Plant Cell. (abstract)

qcML: an exchange format for quality control metrics from mass spectrometry experiments. Mol Cell Proteomics.
Walzer, M., Pernas, LE., Nasso, S., Bittremieux, W., Nahnsen, S., Kelchtermans, P., Pichler, P., van den Toorn, HW., Staes, A., Vandenbussche, J., Mazanek, M., Taus, T., Scheltema, RA., Kelstrup, CD., Gatto, L., van Breukelen, B., Aiche, S., Valkenborg, D., Laukens, K., Lilley, KS., Olsen, JV., Heck, AJ., Mechtler, K., Aebersold, R., Gevaert, K., Vizcaíno, JA., Hermjakob, H., Kohlbacher, O., Martens, L. (2014).  13(8):1905-13 (abstract)

Jagunal homolog 1 is a critical regulator of neutrophil function in fungal host defense.
Wirnsberger, G., Zwolanek, F., Stadlmann, J., Tortola, L., Liu, SW., Perlot, T., Järvinen, P., Dürnberger, G., Kozieradzki, I., Sarao, R., De Martino, A., Boztug, K., Mechtler, K., Kuchler, K., Klein, C., Elling, U., Penninger, JM. (2014). Nat Genet. 46(9):1028-33 (abstract

2013

Frese, CK., Zhou, H., Taus, T., Altelaar, AF., Mechtler, K., Heck, AJ., Mohammed, S. (2013). Unambiguous phosphosite localization using electron-transfer/higher-energy collision dissociation (EThcD). J Proteome Res. 12(3):1520-5 (abstract)

Ivanov, AR., Colangelo, CM., Dufresne, CP., Friedman, DB., Lilley, KS., Mechtler, K., Phinney, BS., Rose, KL., Rudnick, PA., Searle, BC., Shaffer, SA., Weintraub, ST. (2013). Interlaboratory studies and initiatives developing standards for proteomics. Proteomics. 13(6):904-9 (abstract)

Malvezzi, F., Litos, G., Schleiffer, A., Heuck, A., Mechtler, K., Clausen, T., Westermann, S. (2013). A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors. EMBO J. (abstract)

Osman, K., Roitinger, E., Yang, J., Armstrong, S., Mechtler, K., Franklin, FC. (2013). Analysis of meiotic protein complexes from Arabidopsis and brassica using affinity-based proteomics. Methods Mol Biol. 990:215-26 (abstract)

Reiter, W., Klopf, E., De Wever, V., Anrather, D., Petryshyn, A., Roetzer, A., Niederacher, G., Roitinger, E., Dohnal, I., Görner, W., Mechtler, K., Brocard, C., Schüller, C., Ammerer, G. (2013). Yeast protein phosphatase 2A-Cdc55 regulates the transcriptional response to hyperosmolarity stress by regulating Msn2 and Msn4 chromatin recruitment. Mol Cell Biol. 33(5):1057-72 (abstract)

Schmidt, A., Ammerer, G., Mechtler, K. (2013). Studying the fragmentation behavior of peptides with arginine phosphorylation and its influence on phospho-site localization. Proteomics. 13(6):945-54 (abstract)

2012

SIMPATIQCO: A Server-Based Software Suite Which Facilitates Monitoring the Time Course of LC-MS Performance Metrics on Orbitrap Instruments.

Pichler, P., Mazanek, M., Dusberger, F., Weilnböck, L., Huber, CG., Stingl, C., Luider, TM., Straube, WL., Köcher, T., Mechtler, K.

J Proteome Res. 11(11):5540-7 (abstract)

 

CENP-T proteins are conserved centromere receptors of the Ndc80 complex.

Schleiffer, A., Maier, M., Litos, G., Lampert, F., Hornung, P., Mechtler, K., Westermann, S.

Nat Cell Biol. 14(6):604-13 (abstract)

 

APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex.

Uzunova, K., Dye, BT., Schutz, H., Ladurner, R., Petzold, G., Toyoda, Y., Jarvis, MA., Brown, NG., Poser, I., Novatchkova, M., Mechtler, K., Hyman, AA., Stark, H., Schulman, BA., Peters, JM.

Nat Struct Mol Biol. 19(11):1116-23 (abstract)

 

Spatiotemporal regulation of Ipl1/Aurora activity by direct Cdk1 phosphorylation.

Zimniak, T., Fitz, V., Zhou, H., Lampert, F., Opravil, S., Mechtler, K., Stolt-Bergner, P., Westermann, S.

Curr Biol. 22(9):787-93 (abstract)

 

Drosophila CPEB Orb2A Mediates Memory Independent of Its RNA-Binding Domain.

Krüttner, S., Stepien, B., Noordermeer, JN., Mommaas, MA., Mechtler, K., Dickson, BJ., Keleman, K.

Neuron. 76(2):383-95 (abstract)

 

Analysis of protein mixtures from whole-cell extracts by single-run nanoLC-MS/MS using ultralong gradients.

Köcher, T., Pichler, P., Swart, R., Mechtler, K. (2012).

Nat Protoc. 7(5):882-90 (abstract)

 

Inter-Homolog Crossing-Over and Synapsis in Arabidopsis Meiosis Are Dependent on the Chromosome Axis Protein AtASY3.

Ferdous M, Higgins JD, Osman K, Lambing C, Roitinger E, Mechtler K, Armstrong SJ, Perry R, Pradillo M, Cuñado N, Franklin FC.

PLoS Genet. 2012 Feb;8(2):e1002507. Epub 2012 Feb 2. (abstract)

2011

Universal and Confident Phosphorylation Site Localization Using phosphoRS.

Taus T, Köcher T, Pichler P, Paschke C, Schmidt A, Henrich C, Mechtler K.

J Proteome Res. 2011 Dec 2;10(12):5354-62.

 

Systematic phosphorylation analysis of human mitotic protein complexes.

Hegemann B, Hutchins JR, Hudecz O, Novatchkova M, Rameseder J, Sykora MM, Liu S, Mazanek M, Lénárt P, Hériché JK, Poser I, Kraut N, Hyman AA, Yaffe MB, Mechtler K, Peters JM.

Sci Signal. 2011 Nov 8;4(198):rs12.

A systematic analysis of Drosophila TUDOR domain-containing proteins identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway factors.

Handler D, Olivieri D, Novatchkova M, Gruber FS, Meixner K, Mechtler K, Stark A, Sachidanandam R, Brennecke J.

EMBO J. 2011 Aug 23;30(19):3977-93. doi: 10.1038/emboj.2011.308.

 

Quantitative phospho-proteomics to investigate the polo-like kinase 1-dependent phospho-proteome.

Grosstessner-Hain K, Hegemann B, Novatchkova M, Rameseder J, Joughin BA, Hudecz O, Roitinger E, Pichler P, Kraut N, Yaffe MB, Peters JM, Mechtler K.

Mol Cell Proteomics. 2011 Nov;10(11):M111.008540. Epub 2011 Aug 21.

 

Spatial exclusivity combined with positive and negative selection of phosphorylation motifs is the basis for context-dependent mitotic signaling.

Alexander J, Lim D, Joughin BA, Hegemann B, Hutchins JR, Ehrenberger T, Ivins F, Sessa F, Hudecz O, Nigg EA, Fry AM, Musacchio A, Stukenberg PT, Mechtler K, Peters JM, Smerdon SJ, Yaffe MB.

Sci Signal. 2011 Jun 28;4(179):ra42.

 

General statistical modeling of data from protein relative expression isobaric tags.

Breitwieser FP, Müller A, Dayon L, Köcher T, Hainard A, Pichler P, Schmidt-Erfurth U, Superti-Furga G, Sanchez JC, Mechtler K, Bennett KL, Colinge J.

J Proteome Res. 2011 Jun 3;10(6):2758-66. Epub 2011 May 11.

 

Proteomic analysis reveals a virtually complete set of proteins for translation and energy generation in elementary bodies of the amoeba symbiont Protochlamydia amoebophila.

Sixt BS, Heinz C, Pichler P, Heinz E, Montanaro J, Op den Camp HJ, Ammerer G, Mechtler K, Wagner M, Horn M.

Proteomics. 2011 May;11(10):1868-92. doi: 10.1002/pmic.201000510. Epub 2011 Apr 18.

 

Ultra-high-pressure RPLC hyphenated to an LTQ-Orbitrap Velos reveals a linear relation between peak capacity and number of identified peptides.

Köcher T, Swart R, Mechtler K.

Anal Chem. 2011 Apr 1;83(7):2699-704. Epub 2011 Mar 9.

 

Quality control in LC-MS/MS.

Köcher T, Pichler P, Swart R, Mechtler K.

Proteomics. 2011 Mar;11(6):1026-30. doi: 10.1002/pmic.201000578. Epub 2011 Feb 7.

 

HSPC117 is the essential subunit of a human tRNA splicing ligase complex.

Popow J, Englert M, Weitzer S, Schleiffer A, Mierzwa B, Mechtler K, Trowitzsch S, Will CL, Lührmann R, Söll D, Martinez J.

Science. 2011 Feb 11;331(6018):760-4.

Improved precision of iTRAQ and TMT quantification by an axial extraction field in an Orbitrap HCD cell.

Pichler P, Köcher T, Holzmann J, Möhring T, Ammerer G, Mechtler K.

Anal Chem. 2011 Feb 15;83(4):1469-74. Epub 2011 Jan 28.

Altered Mascot search results by changing the m/z range of MS/MS spectra: analysis and potential applications.

Köcher T, Pichler P, Mazanek M, Swart R, Mechtler K.

Anal Bioanal Chem. 2011 Jun;400(8):2339-47. Epub 2010 Dec 15.

2010

Proteomic analysis of endosomes from genetically modified p14/MP1 mouse embryonic fibroblasts.

Stasyk T, Holzmann J, Stumberger S, Ebner HL, Hess MW, Bonn GK, Mechtler K, Huber LA.

Proteomics. 2010 Nov;10(22):4117-27. doi: 10.1002/pmic.201000258. Epub 2010 Nov 2. PMID: 21080497

 

Lesson from the stoichiometry determination of the cohesin complex; a short protease mediated elution increases the recovery from crosslinked antibody-conjugated beads.

Holzmann J, Fuchs J, Pichler P, Peters JM, Mechtler K.

J Proteome Res. 2010 Nov 3. PMID: 21043528

 

Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4.

Rumpf C, Cipak L, Schleiffer A, Pidoux A, Mechtler K, Tolic'-Nørrelykke IM, Gregan J.

Cell Cycle. 2010 Oct 1;9(19):3997-4004. Epub 2010 Oct 3. PMID: 20935472

 

An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila.

Olivieri D, Sykora MM, Sachidanandam R, Mechtler K, Brennecke J.

EMBO J. 2010 Oct 6;29(19):3301-17. Epub 2010 Sep 3.PMID: 20818334

 

Live-cell imaging RNAi screen identifies PP2A-B55alpha and importin-beta1 as key mitotic exit regulators in human cells.

Schmitz MH, Held M, Janssens V, Hutchins JR, Hudecz O, Ivanova E, Goris J, Trinkle-Mulcahy L, Lamond AI, Poser I, Hyman AA, Mechtler K, Peters JM, Gerlich DW.

Nat Cell Biol. 2010 Sep;12(9):886-93. Epub 2010 Aug 15.PMID: 20711181

 

Peptide labeling with isobaric tags yields higher identification rates using iTRAQ 4-plex compared to TMT 6-plex and iTRAQ 8-plex on LTQ Orbitrap.

Pichler P, Köcher T, Holzmann J, Mazanek M, Taus T, Ammerer G, Mechtler K.

Anal Chem. 2010 Aug 1;82(15):6549-58.PMID: 20593797

 

Casein kinase 1 is required for efficient removal of Rec8 during meiosis I.

Rumpf C, Cipak L, Dudas A, Benko Z, Pozgajova M, Riedel CG, Ammerer G, Mechtler K, Gregan J.

Cell Cycle. 2010 Jul 21;9(13). PMID: 2058146

 

Raman microspectroscopy reveals long-term extracellular activity of Chlamydiae.

Haider S, Wagner M, Schmid MC, Sixt BS, Christian JG, Häcker G, Pichler P, Mechtler K, Müller A, Baranyi C, Toenshoff ER, Montanaro J, Horn M.

Mol Microbiol. 2010 Aug;77(3):687-700. Epub 2010 Jun 1.PMID: 20545842

 

Peptide orientation affects selectivity in ion-exchange chromatography.

Alpert AJ, Petritis K, Kangas L, Smith RD, Mechtler K, Mitulovi? G, Mohammed S, Heck AJ.

Anal Chem. 2010 Jun 15;82(12):5253-9.PMID: 20481592

 

Topology and organization of the Salmonella typhimurium type III secretion needle complex components.

Schraidt O, Lefebre MD, Brunner MJ, Schmied WH, Schmidt A, Radics J, Mechtler K, Galán JE, Marlovits TC.

PLoS Pathog. 2010 Apr 1;6(4):e1000824.PMID: 20368966

 

Systematic analysis of human protein complexes identifies chromosome segregation proteins.

Hutchins JR, Toyoda Y, Hegemann B, Poser I, Hériché JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM.

Science. 2010 Apr 30;328(5978):593-9. Epub 2010 Apr 1.PMID: 20360068

 

Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis.

Katis VL, Lipp JJ, Imre R, Bogdanova A, Okaz E, Habermann B, Mechtler K, Nasmyth K, Zachariae W.

Dev Cell. 2010 Mar 16;18(3):397-409.PMID: 20230747

 

QIKS--Quantitative identification of kinase substrates.

Morandell S, Grosstessner-Hain K, Roitinger E, Hudecz O, Lindhorst T, Teis D, Wrulich OA, Mazanek M, Taus T, Ueberall F, Mechtler K, Huber LA.

Proteomics. 2010 May;10(10):2015-25.PMID: 20217869

 

Probing the phosphoproteome of HeLa cells using nanocast metal oxide microspheres for phosphopeptide enrichment.

Leitner A, Sturm M, Hudecz O, Mazanek M, Smått JH, Lindén M, Lindner W, Mechtler K.

Anal Chem. 2010 Apr 1;82(7):2726-33.PMID: 20201521

 

Reducing the haystack to find the needle: improved protein identification after fast elimination of non-interpretable peptide MS/MS spectra and noise reduction.

Mujezinovic N, Schneider G, Wildpaner M, Mechtler K, Eisenhaber F.

BMC Genomics. 2010 Feb 10;11 Suppl 1:S13.PMID: 20158870

 

A Syd-1 homologue regulates pre- and postsynaptic maturation in Drosophila.

Owald D, Fouquet W, Schmidt M, Wichmann C, Mertel S, Depner H, Christiansen F, Zube C, Quentin C, Körner J, Urlaub H, Mechtler K, Sigrist SJ.

J Cell Biol. 2010 Feb 22;188(4):565-79.PMID: 20176924

 

A new acid mix enhances phosphopeptide enrichment on titanium- and zirconium dioxide for mapping of phosphorylation sites on protein complexes.

Mazanek M, Roitinger E, Hudecz O, Hutchins JR, Hegemann B, Mitulovi? G, Taus T, Stingl C, Peters JM, Mechtler K.

J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Feb 15;878(5-6):515-24. Epub 2009 Dec 22.PMID: 20075017

 

2009

Kiermaier, E., Woehrer, S., Peng, Y., Mechtler, K., and Westermann, S. (2009). A Dam1-based artificial kinetochore is sufficient to promote chromosome segregation in budding yeast. Nat Cell Biol 11, 1109-1115.

 

Kocher, T., Pichler, P., Schutzbier, M., Stingl, C., Kaul, A., Teucher, N., Hasenfuss, G., Penninger, J.M., and Mechtler, K. (2009). High precision quantitative proteomics using iTRAQ on an LTQ Orbitrap: a new mass spectrometric method combining the benefits of all. J Proteome Res 8, 4743-4752.

 

Holzmann, J., Pichler, P., Madalinski, M., Kurzbauer, R., and Mechtler, K. (2009). Stoichiometry determination of the MP1-p14 complex using a novel and cost-efficient method to produce an equimolar mixture of standard peptides. Anal Chem 81, 10254-10261.

 

Zimniak, T., Stengl, K., Mechtler, K., and Westermann, S. (2009). Phosphoregulation of the budding yeast EB1 homologue Bim1p by Aurora/Ipl1p. J Cell Biol 186, 379-391.

 

Cipak, L., Spirek, M., Novatchkova, M., Chen, Z., Rumpf, C., Lugmayr, W., Mechtler, K., Ammerer, G., Csaszar, E., and Gregan, J. (2009). An improved strategy for tandem affinity purification-tagging of Schizosaccharomyces pombe genes. Proteomics 9, 4825-4828.

 

Brehme, M., Hantschel, O., Colinge, J., Kaupe, I., Planyavsky, M., Köcher, T., Mechtler, K., Bennett, KL., Superti-Furga, G. (2009). Charting the molecular network of the drug target Bcr-Abl. Proc Natl Acad Sci U S A. 106(18):7414-9abstract) (fulltext)

 

Fuhrmann, J., Schmidt, A., Spiess, S., Lehner, A., Turgay, K., Mechtler, K., Charpentier, E., Clausen, T. (2009). McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR. Science. 324(5932):1323-7 (abstract) (fulltext)

 

Herzog, F., Primorac, I., Dube, P., Lenart, P., Sander, B., Mechtler, K., Stark, H., Peters, JM. (2009). Structure of the anaphase-promoting complex/cyclosome interacting with a mitotic checkpoint complex. Science. 323(5920):1477-81abstract) (fulltext)

 

Leitner, A., Sturm, M., Smått, JH., Järn, M., Lindén, M., Mechtler, K., Lindner, W. (2009). Optimizing the performance of tin dioxide microspheres for phosphopeptide enrichment. Anal Chim Acta. 638(1):51-7 (abstract) (fulltext)

 

Müller, MQ., de Koning, LJ., Schmidt, A., Ihling, C., Syha, Y., Rau, O., Mechtler, K., Schubert-Zsilavecz, M., Sinz, A. (2009). An innovative method to study target protein-drug interactions by mass spectrometry. J Med Chem. 52(9):2875-9abstract) (fulltext)

 

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