Immunoprecipitation protocol for mass spectrometry

Gel Blum silver staining protocol

Manual - How to read your mass spectrometry result

Protein precipitation protocol with Acetone

Cells lysis in 8M urea protocol for mass spectrometric analysis

Standard digestion procedures

Nuclear extracts

  REAP Suzuki et al

In order to make the protocol MS compatible we advise to add an additional centrifugation step of the nuclear pellets through a 10% sucrose cushion.