Equipment

The LTQ Orbitrap Velos combines the industry-leading Orbitrap mass analyzer, a new Higher-Energy Collisional Dissociation (HCD) cell and the new LTQ Velos, the world's fastest and most sensitive ion trap mass spectrometer, to routinely deliver ultra-high resolution and accurate mass data.

The instruments supports a wide range of applications and combines three different and complementary fragmentation techniques CID, HCD, and ETD. With the DDDT (Data Dependent Decision Tree) technology, the high scan rate and the accurate mass measurement (<1 ppm in full scan) we use this Instrument for peptide sequencing, PTM mapping and quantification.

It is coupled to a Dionex UltiMate 3000 Rapid Separation LC (RSLC) system. Mainly operated in reversed phase mode with C18 columns.

The LTQ Orbitrap Velos combines the industry-leading Orbitrap mass analyzer, a new Higher-Energy Collisional Dissociation (HCD) cell and the new LTQ Velos, the world's fastest and most sensitive ion trap mass spectrometer, to routinely deliver ultra-high resolution and accurate mass data.

The instruments supports a wide range of applications and combines different and complementary fragmentation techniques like CID and HCD.

It is coupled to a Dionex UltiMate 3000 dual gradient system, mainly operated in reversed phase mode with C18 columns.

The LTQ-Velos ion trap mass spectrometer is one of the fastest and most sensitive ion trap mass spectrometer allowing the optimal analysis of complex peptide samples. Reliable sequencing and analysis of post translational modifications are established through multiple fragmentation procedures.

The LTQ-Velos is coupled to a Dionex Ultimate 3000 dual gradient system, which operates in reversed phase mode with C18 columns.

Based on the fast Thermo Scientific linear ion trap and the Orbitrap technology, the LTQ Orbitrap XL hybrid FTMS (Fourier Transform Mass Spectrometer) supports a wide range of applications and combines three different and complementary fragmentation techniques CID, HCD, and ETD. With the DDDT (Data Dependent Decision Tree) technology, the high scan rate and the accurate mass measurement (<1 ppm in full scan) we use this Instrument for peptide sequencing, PTM mapping and quantification.

It is coupled to an U3000 Dual Gradient reversed phase nano-HPLC from Dionex. Basically we use 25 cm PepMap C18 columns together with C18 precolumns and a triple phase gradient.

The QSTAR Elite Hybrid system from Applied Biosystems is a quadrupol-TOF hybrid instrument that is equipped with an electrospray ion source which allows direct coupling to an UltimateII nanoLC-system from Dionex. The instrument offers high mass accuracy across a broad mass range - without the low-mass cut-off of ion-traps. Therefore, the QSTAR is ideally suited for quantification of iTRAQ labeled samples. The acquired data can be analyzed with the ProteinPilot software package which employs one of the most advanced protein grouping algorithms - a feature that is highly important when analyzing and quantifying protein isoforms. Another application is the analysis of intact proteins, both, by direct infusion and by LC using a monolithic column. Recently, the QSTAR was equipped with an IonCooler device, which increases its sensitivity for the analysis of protein complexes of 0.5MDa and above.

This MALDI-TOF instrument is used for the quality control of the peptides, which are synthesized in our facility.

This SCX-HPLC system uses SCX columns produced by Dionex (1mm/25cm) and is used for method development and complex sample fractionation.

This SCX-HPLC system uses SCX columns produced by Dionex (1mm/25cm) and is used for method development and complex sample fractionation.

This instrument is used for sample fractionation via SCX or ERLIC methods. It is also equipped with a PROBOT spotter for MALDI target spotting.

This system is equipped with a reversed phase monolithic column and is used for sample pre-screening and peptide/protein separation.

With its PROBOT spotter, this system is mainly used for MALDI target spotting.

Our "old lady" is basically used for column testing, method development and sample pre-screening.