Microscopy

Before using any of the microscopes, a training by the BioOptics Facility staff is needed.
Users do not train other users!
Please schedule an appointment with Gabi Stengl (0664 88464463) for wide-field imaging or Pawel Pasierbek (0664 88464460) for confocal and live-cell imaging.

The microscopes can be reserved online  through the Online Booking System GRM3 - grm3.imp.univie.ac.at.
Sign-Up is on a first come, first serve basis.
Appointments that cannot be held must be cancelled as early as possible in order to free up the slots for other users.
If the user does not show up within 30 minutes after the start of the booking-time the whole reservation is considered cancelled and the equipment is available to everybody.

Confocal System shut-down:

• After you finish your work clean the objectives from immersion medium with the lens cleaning tissue - don´t use it to clean the slides since it is expensive! Remove the rest of the oil with the cotton stab submerged in the "Wundbenzin".
• Change the objective to 10x.
• Check in the booking system when is the next person coming. If you are the last one (or next slot is schedule in more than 30 min.) switch-off the lasers and the fluorescence light source.
• Remove your samples and clean the working area.
• Report any problems immediately to BioOptics Staff.

Widefield microscope shut-down:

• After you finish your work clean the objectives from immersion medium with the lens cleaning tissue - don´t use it to clean the slides since it is expensive!
• Change the objective to 10x.
• Check in the booking system when the next person is coming. If you are the last one (or next slot is schedule in more than 30 min.) switch-off the fluorescence light source.
• Remove your samples and clean the working area.
• Report any problems immediately to Biooptics Staff.

All data should be stored on server space! Data stored on local hard disks can be deleted at any time without notice.

If you encounter any problems with the equipment, please inform BioOptics staff as soon as possible even if the problem has been resolved.
If you have any questions concerning the operation of the machines or the planning and execution of experiments do not hesitate to ask the BioOptics staff for assistance.