Flow Cytometry

Training

Experiments involving flow cytometry should be planned and discussed ahead with the BioOptics Facility staff. Before using any of the analytical machines (FACS Canto, FACS Fortessa, Guava Plate Reader), a training by the BioOptics Facility staff is compulsory prior to usage.
Users do not train other users!

If you cannot remember how to operate a system you were already introduced to, please do not hesitate to ask the BioOptics team for advice.

Please schedule an appointment via facs@imp.ac.at at least 3 days in advance to receive such a training.

Because the machines differ in software and features, separate training is necessary for each machine.

 

 

Sign-Up

The analytical machines (FACS Canto, FACS Fortessa, Guava Plate reader) can be reserved through the Online Booking system GRM3 - http://grm3.imp.univie.ac.at/. Sign-Up is on a first come, first serve basis.

If the user does not show up within 30 minutes after the start of the booking-time the whole reservation is cancelled and the equipment is available to everybody.

In GRM you can also sign up to receive an Email, if a booking on a chosen analyser is deleted or shortened and therefor additional instrument-time is available.

To allow proper planning for everybody, we would strongly ask you to well plan your experiments, only book the time you actually need and cancel or shorten your bookings as soon as you find out that plans have changed!

Cell sorters may be booked via the Sort Operators. Please visit us at the FACS Room (1st floor, IMP Building) or write an email to facs(at)imp.ac.at.

Clean-Up and Shut-Down

Users are responsible to clean up after completion of their measurements. Please follow the respective shutdown and cleaning procedures of the instrument in our protocols section.
The last user is asked to power-down the system at the end of the day (check the booking system!).

Data Storage

All data should be stored on server space! Data stored on local hard disks can be deleted at any time without notice.

Acknowledgement

If you publish work supported by the BioOptics Facility, e.g. by using one or more analysers or support in cell sorting as well as in data analysis, please do not forget to acknowledge the facility in your paper.

General

If you encounter any problems with the equipment, please inform BioOptics staff as soon as possible even if the problem has been resolved.
If you have any questions concerning the operation of the machines or the planning and execution of experiments do not hesitate to ask the BioOptics staff for assistance.