ImageJ/Fiji
ImageJ/Fiji is a free software platform suited for a variety of different imaging tasks. We provide advice in usage and analytical questions, as well as automated workflows in Fiji Macro Language for batch analyses. In regular intervals we give three day courses on Image analysis using Fiji to provide users with the tools for solving problems on their own.
Selection of projects already done
Alignment from grid in TIRF images
Align two channels from TIRF acquisition projected onto a single sensor.
More information can be found here (PDF).
Elispot count and size measurement
Count number of Spots per dish and determine spot sizes.
More information can be found here (PDF).
Expression in apoptotic cells on DIC images
Quantification of apoptotic cells
More information can be found here (PDF).
Quantification of Apical ZO - 1
Automated analysis of 2D projections to quantify sizes, area of closed holes and branching of cells.
More information can be found here (PDF).