M42 – LSM980 Axio Observer (inverted)

System Description

 

The M42 system is an inverted point laser scanning confocal microscope – LSM980. It is a spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. LSM980 features 4 highly sensitive GaAsP (Gallium Arsenide) detectors with a QE of 45% and up to 2x SNR as compared to standards PMTs. 

The system is equipped with a motorized xyz-stage and a definite focus (hardware autofocus device). The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.  

This microscope is controlled with the ZEN Blue software.

Widefield Illumination

LED  

LED for brightfield

Lasers for Confocal Imaging

 

405nm

445nm

488nm

514nm

561nm

639nm

Filters

 

CH1: 380-736 
CH2: 380-736 
CHS1-4 GaAsP: 409-694  
CHD: T-PMT (transmitted light)  

Objectives

10x/0.3 EC plan-neofluar DIC
20x/0.8 plan-apochromat DIC
25x/0.8 LD LCI plan-apochromat DIC

40x/1.1 LD C-Apochromat Water
40x/1.4 EC plan-apochromat Oil DIC
63x/1.4 plan-apochromat Oil DIC

Additional Devices

 

Incubation with CO2 
XY Motorized Stage 
Piezo Z stage insert 
Multiple Position Acquisition 
High Dynamic Range acquisition (HDR) 
FRAP 
Tiles

Reservation

orgt.vbc.ac.at