M42 – LSM980 Axio Observer (inverted)
System Description
The M42 system is an inverted point laser scanning confocal microscope – LSM980. It is a spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. LSM980 features 4 highly sensitive GaAsP (Gallium Arsenide) detectors with a QE of 45% and up to 2x SNR as compared to standards PMTs.
The system is equipped with a motorized xyz-stage and a definite focus (hardware autofocus device). The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.
This microscope is controlled with the ZEN Blue software.
Widefield Illumination
LED
LED for brightfield
Lasers for Confocal Imaging
405nm
445nm
488nm
514nm
561nm
639nm
Filters
CH1: 380-736
CH2: 380-736
CHS1-4 GaAsP: 409-694
CHD: T-PMT (transmitted light)
Objectives
10x/0.3 plan-apochromat DIC
20x/0.8 plan-apochromat DIC
25x/0.8 LD LCI plan-apochromat DIC
40x/1.1 LD C-Apochromat Water
40x/1.3 EC plan-apochromat Oil DIC
63x/1.4 plan-apochromat Oil DIC
Additional Devices
Incubation with CO2
XY Motorized Stage
Piezo Z stage insert
Multiple Position Acquisition
High Dynamic Range acquisition (HDR)
FRAP
Tiles