M41 – LSM980 Axio Observer (inverted) with Airyscan II and incubation

System Description

The M41 system is an inverted point laser scanning confocal microscope – LSM980. It is a spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. LSM980 features 4 highly sensitive GaAsP (Gallium Arsenide) detectors with a QE of 45% and up to 2x SNR as compared to standards PMTs. 

The system is equipped with a motorized xyz-stage and a definite focus (hardware autofocus device). The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.  

In addition the M41 system features Airyscan II - a hexagonal GaAsP detector. This device enables improvement of the resolving power in XYZ by a factor of 1.7. The fast mode of the AiryScan called AiryScan MPLX allows 4-8x faster acquisition with up to 1.5x improvement of the optical resolving power and up to 4x increase of signal-to-noise ratio (SNR) compared to 1AU confocal mode. 

This microscope is controlled with the ZEN Blue software.

Widefield Illumination

LED  

LED for brightfield

Lasers for Confocal Imaging

405nm

445nm

488nm

514nm

561nm

639nm

Filter

CH1: 380-736 
CH2: 380-736 
CHS1-4 GaAsP: 409-694  
CHD: T-PMT (transmitted light)  

 

Splitter to AiryScan Detector: SP 505; LP 525; LP570; SP 550; LP460; SP 615; Plate; Mirror 

AIRYSCAN: BP 420-480+BP495-550;  BP 420-480+BP570-630;   BP 420-480+LP605;   BP 465-505+BP525-585;   BP 495-550+BP570-630;   BP 495-555+LP660; BP 570-620+LP 660

Objectives

10x/0.3 EC plan-neofluar
40x/1.3 EC plan-neofluar Oil DIC 
63x/1.4 plan-apochromat Oil DIC

Additional Devices

Incubation with CO2 
XY Motorized Stage 
Piezo Z stage insert 
Multiple Position Acquisition 
High Dynamic Range acquisition (HDR) 
FRAP 
Tiles