M40 - Spinning Disk Confocal Olympus (inverted) with incubation

System Description

The M40 Olympus spinning disk confocal is based on an Olympus IX3 Series (IX83) inverted microscope, equipped with a dual-camera Yokogawa W1 spinning disk (SD) allowing fast confocal acquisition. All components are controlled by the CellSense software.

The system is able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. There is also Olympus Z-Drift Compensation (ZDC) hardware autofocus system for long-term stabilization.

This makes it suitable e.g. for various live experiments or the automatic acquisition of a large amount of images (screening).

Illumination

LED: 400nm, 465nm, 565nm

Transmitted Light LED illumination

Lasers for SD

405nm

488nm

561nm

640nm

Filters

Filters Vis:

Ex 365/50; Em LP 397; BSP 395

Ex 480/20; Em 530/40; BSP 495

Ex 540/20; Em LP575; BSP 570

Ex 631/23; Em 700/50; BSP 660


Filters SD-Emission:

 

Camera 1:

447/60

525/50

617/73

685/40

 

Camera 2:

447/60

525/50

Dichroic for spinning disk

405/488/568/647

Objectives

4x/0.16 UPLSApo WD -13mm

10x/0.4 UPLSApo WD – 3.1mm

10x/0.3 UPLFApo WD –10mm

20x/0.75 UPLSApo WD – 0.6mm

40x/0.75 UPLFLN WD – 0.5mm

40x/1.25 Silicon Oil UPLSApo WD – 0.3mm

 

On request

 

30x/1.05 Silicon Oil UPLSApo WD – 0.8mm

40x/1.4 Oil UPLXApo WD – 0.18mm

60x/1.42 Oil UPLXApo WD – 0.15mm

100x/1.45 Oil UPLXApo WD – 0.13mm

Camera

Camera 1 and 2 – Hamamatsu Orca Flash 4.0, Pixel Size: 6.5μm

Additional magnification 3.2x

Additional devices

Motorized XY stage with piezo Z

Hardware Autofocus

Incubatior and CO2