M33 - LSM 880 Axio Observer (inverted) with Airyscan /Airyscan fast and incubation and 40x/1.2 W, Glycerol AutoCorr objective

System Description

The M33 system is an inverted point laser scanning confocal microscope (LSM880).It is a complete spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. LSM880 features a highly sensitive array of 32 GaAsP (Gallium Arsenide) detectors with a QE of 45% and up to 2x SNR as compared to standards PMTs.

The system is equipped with a motorized xyz-stage and a definite focus (hardware autofocus device). The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.

In addition the M33 system features Airyscan - a hexagonal GaSaP detector. This device enables improvement of the resolving power in XYZ by a factor of 1.7. The Airyscan fast option allows 4x faster acquisition with up to 1.5x improvement of the optical resolving power and 4x increase of signal-to-noise ratio (SNR) compared to 1AU confocal mode.

 

 

Widefield Illumination

LED

LED for brightfield

Lasers for Confocal Imaging

Laser Diode 405 25mW

Argon 458, 488, 514 30mW

DPSS 561 15mW

HeNe 594 2mW

HeNe 633 5mW

Filter

CH1: 371-735
CH2: 479-735
CH3 Quasar (GaAsP)
 : 416-696 in 8.3nm steps
CHD
: T-PMT (transmitted light)

AIRYSCAN: BP 420-480+BP495-550;  BP 420-480+BP495-620;   BP 420-480+LP605;   BP 465-505+LP525;   BP 495-550+LP570;   BP 570-620+LP645

Objectives

10x/0.45 plan-apochromat

20/0.8 plan-apochromat,

40x/1.3 EC plan-neofluar Oil DIC

LD LCI 40x/1.2 Water, Glycerol DIC AutoCorr

63x/1.4 plan-apochromat Oil DIC

Additional Devices

 

Incubation with CO2
XY Motorized Stage
Piezo Z stage insert
Multiple Position Acquisition
High Dynamic Range acquisition (HDR)
FRAP
Tiles