M27 - LSM 880 Axio Observer (inverted) with Airyscan and incubation

System Description

The M27 system is an inverted point laser scanning confocal microscope (LSM880).It is a complete spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. LSM880 features a highly sensitive array of 32 GaAsP (Gallium Arsenide) detectors with a QE of 45% and up to 2x SNR as compared to standards PMTs. The system is equipped with a motorized xyz-stage and a definite focus (hardware autofocus device). The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.In addition the M27 system features Airyscan - a hexagonal GaSaP detector. This device enables improvement of the resolving power in XYZ by a factor of 1.7.

 

 

Widefield Illumination

LED

LED for brightfield

Lasers for Confocal Imaging

Laser Diode 405 25mW

Argon 458, 488, 514 30mW

DPSS 561 15mW

HeNe 633 5mW

Filters

CH1: 371-740
CH2
: 479-750
CH3 Quasar (GaAsP)
 : 416-728 in 8.3nm steps
CHD
: T-PMT (transmitted light)

AIRYSCAN: BP 420-480+BP495-550;  BP 420-480+BP495-620;   BP 420-480+LP605;   BP 465-505+LP525;   BP 495-550+LP570;   BP 570-620+LP645

Objectives

10x/0.45 plan-apochromat

20/0.8 plan-apochromat,

40x/1.4 EC plan-apochromat Oil DIC

63x/1.4 plan-apochromat Oil DIC

100x/1.46 alpha plan-apochromat Oil DIC

Additional Devices

XY Motorized Stage
Piezo Z stage insert
Multiple Position Acquisition
High Dynamic Range acquisition (HDR)
Physiology Macro