M26 - LSM780 Axio Observer (inverted)

System Description

The M26 system is an inverted point laser scanning confocal microscope (LSM780). It is a complete spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. LSM780 features high sensitive array of 32 GaAsP (Gallium Arsenide) detectors with QE of 45% and up to 2x SNR.
The system is equipped with a motorized xy-stage and definite focus (hardware autofocus device).
The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse. 

 

 

Widefield Illumination

LED
Halogen Lamp 50W 

Lasers for Confocal Imaging

Laser Diode 405 25mW
Argon 458, 488, 514 30mW
DPSS 561 15mW
HeNe 633 5mW

Filters

CH1: 371-735
CH2: 479-735
CH3 Quasar (GaAsP) : 416-690 in 8.3nm steps
CHD
: T-PMT (transmitted light)

Objectives

10x/0.3 EC plan-neofluar
20/0.8 plan-apochromat,
40x/1.4 EC plan-apochromat Oil DIC
63x/1.4 plan-apochromat Oil DIC

Additional Devices

XY Motorised Stage, Piezo Z stage insert, Multiple Position Acquisition, High Dynamic Range acquisition (HDR), Physiology Macro