M24 - LSM780 Axio Imager (upright)

System Description

The M24 system is an upright point laser scanning confocal microscope (LSM780). It is a complete spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. Designed for live cell imaging, LSM780 features high sensitive array of 32 GaAsP (Gallium Arsenide) detectors with QE of 45% and up to 2x SNR.
The system is equipped with a motorized xy-stage, it´s fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.

Widefield Illumination

LED – 400nm, 465nm, 555nm
Halogen Lamp 50W

Lasers for Confocal Imaging

Laser Diode 405 25mW
Argon 458, 488, 514 30mW
DPSS 561 15mW
HeNe 633 5mW

Filters

CH1: 371-735
CH2: 479-735
CH3 Quasar (GaAsP) : 416-690 in 8.3nm steps
CHD
: T-PMT (transmitted light)

 

 

Objectives

10x/0.3 EC plan-neofluar
20x/0.8 plan-Apochromat
25x/0.8 LD LCI plan-apochromat, multiimmersion DIC
40x/1.3 EC plan-neofluar Oil DIC
63x/1.4 plan-apochromat Oil DIC

 

Water dipping objectives for live-cell imaging

40x/0.75W Achroplan
63x/0.9W Achroplan
100x/1.0W Achroplan

Additional Devices

XY Motorised Stage, Multiple Position Acquisition.