M21 - LSM 710 2P

System Description

The M21 system is an inverted point laser scanning confocal microscope (LSM710). It is a complete spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. The system is also equipped with the Chameleon 2-Photon laser and the non-descaned detectors (NDD) as well as a motorized xy-stage.

The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-Stacks, time-lapse.

This makes it suitable e.g. for a xyzt-expermient on up to 4 slides, using a special stage insert and using the 2-Photon Laser it is especially suitable for deep tissue imaging.

Widefield Illumination

Short Arc Lamp 120W
Halogen Lamp 50W

Lasers for Confocal Imaging

Laser Diode 405 25mW
Argon 458, 488, 514 30mW
DPSS 561 15mW
HeNe 633 5mW
Chameleon 2Pi - 705-980nm

Filters

CH1: 371-740
CH2
: 479-750
CH3 Quasar
:416-728 in 10nm steps
CHD
: T-PMT (transmitted light)
NDD PMT
:
1. BP500-550, BS LP555
2. BP565-610, Mirror

Objectives

10x/0.3 EC plan-neofluar
25/0.8 LD LCI plan-apochromat, multiimmersion DIC
40x/1.3 EC plan-neofluar Oil DIC
63x/1.4 plan-apochromat Oil DIC

Additional Devices

Small incubation Chamber with temperature and CO2 control, XY Motorised Stag, Multipile Position Acquisition.