M20 - TIRF 3 System Axio Observer (inverted) with incubation

System Description

The M20 system is an inverted widefield microscope for brightfield and fluorescence. It is equipped with temperature, CO2 and humidity control, a motorized xyz-stage, an EMCCD camera and in addition with a motorized TIRF (Total Internal Reflection Fluorescence) insert allowing high resolution visualization of structures within 100nm of the coverslip. All components are controlled by Axiovision. The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths including brightfield (DIC), z-Stacks, time-lapse. Using the TIRF insert is especially useful for visualization of membrane bound components.

Illumination

Short Arc Lamp 120W
Halogen Lamp 50W

Lasers for TIRF

405nm 50mW
488nm 100mW
561nm 40mW
639nm 30mW

Filters

Ex 488/10nmEm 525/50nm
Ex 406+493+561nmEm 460+525+608nm
Ex 483+564+642nmEm 526+601+688nm
Em 525/50nm
Em 600/50nm
Em 692/10nm
Em 700/75nm

Objectives

10x/0.32 EC plan-neofluar Ph1 DIC I
63x/1.4 plan-apochromat Oil DIC III
100x/1.46 alpha plan-apochromat Oil DIC III

Additional Devices

Incubation chamber with temperature and CO2 control
motorized XY stage