
M09 - LSM780 Axio Observer (inverted) with incubation
System Description

The M09 system is an inverted point laser scanning confocal microscope (LSM780). It is a complete spectral system allowing the free choice of detection and the acquisition of lambda-stacks as well as subsequent unmixing for the separation of dyes with overlapping emission spectra. LSM780 features high sensitive array of 32 GaAsP (Gallium Arsenide) detectors with QE of 45% and up to 2x SNR.
The system is equipped with a motorized xy-stage and definite focus (hardware autofocus device).
The system is fully programmable and therefore able to run multidimensional experiments including any combination of the following: multiple positions, multiple wavelengths, z-stacks, time-lapse.
Widefield Illumination
LED 365nm, 425nm, 470nm, 565nm
White Light LED
Lasers for Confocal Imaging
Laser Diode 405 25mW
Argon 458, 488, 514 30mW
DPSS 561 15mW
HeNe 633 5mW
Filters
CH1: 371-740
CH2: 479-750
CH3 Quasar (GaAsP) : 416-728 in 8.3nm steps
CHD: T-PMT (transmitted light)
Objectives
10x/0.3 EC plan-neofluar
25/0.8 LD LCI plan-apochromat, multiimmersion DIC
40x/1.3 EC plan-neofluar Oil DIC
63x/1.4 plan-apochromat Oil DIC
Additional Devices
XY Motorised Stage, Piezo Z stage insert, Definite Focus, Multipile Position Acquisition.