M05 - Axio Imager.Z2 (upright) with sCMOS camera and Apotome2

System Description

The M05 system run under Zen Blue, is an upright widefield microscope equipped with transmitted and fluorescent light source. It can be used to acquire multicolor fluorescent images of fixed samples, as well as phase contrast (Ph) and differencial interference contrast (DIC) images.

Additionally the system is equipped with Apotome2 - a structure illumination imaging technology that combines the advantages of WF (very good contrast AND speed) and confocal (optical sectioning).
The final image is calculated out of 3 images acquired in the structured illumination mode.
This technique can be applied to all fluorophores and a wide range of specimens.

A motorized XY stage allows multiposition experiments.

Widefield Illumination

Sola SM2

White Light LED

Filters

Ex 406/15nm   Em 457/50nm
Ex 436/25nm   Em 480/40nm
Ex 470/40nm   Em 525/50nm
Ex 480/40nm   Em 510LP
Ex 500/20nm   Em 535/30nm
Ex 545/30nm   Em 610/75nm
Ex 560/55nm   Em 645/75nm
Ex 620/60nm   Em 700/70nm

Objectives

5x/0.15 plan-neofluar
10x/0.3 plan-neofluar
20/0.8 plan-apochromat
40x/1.3 plan-neofluar Oil
63x/1.4 plan-apochromat Oil DIC
100x/1.45 alpha-plan-fluar Oil

Camera

Hamamatsu Orca Flash 4

6.5μm pixel

Additional Devices

motorized XY stage

Apotome2 structured illumination